Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023228_byup CCTGGTGTATGGGGCCGGGGATGGGGGTCCTG(+ATATAT)GATCCCTGCACTCCCCATGCTCCAAGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240607023227_4f8h CTTGACAGGGCTTGGAGCATGGGGAGTGCAGG(+ATATAT)GATCCAGGACCCCCATCCCCGGCCCCATACA NGG 10 8 17 8 24 0 100 5 10
    240607023226_w1li CCGATACACCAGGTCATTCCTCTGCCCACTTG(+ATATAT)GTACCTGGTGTACTAAAGGACTGCATGTCTT NGG 10 8 17 8 24 0 100 5 10
    240607023224_w4k9 CTGCAAAGACATGCAGTCCTTTAGTACACCAG(+ATATAT)GTACCAAGTGGGCAGAGGAATGACCTGGTGT NGG 10 8 17 8 24 0 100 5 10
    240607023222_ztb1 CTTGACAGGGCCTGGCGCATGGGGAGTGCAGG(+ATATAT)GATCCAGGACCCCCATCCCCAACCCGATACA NGG 10 8 17 8 24 0 100 5 10
    240607023220_w74e CCTGGTGTATCGGGTTGGGGATGGGGGTCCTG(+ATATAT)GATCCCTGCACTCCCCATGCGCCAGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240607023219_e7ky ACTCCCCATGCTCCAGGCCCTGTCAAGTGCAG(+ATATAT)GTACCTGCACTAACTCCAGCATTTCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240607023218_kjd4 TCAGCTGCAGTGAAATGCTGGAGTTAGTGCAG(+ATATAT)GTACCTGCACTTGACAGGGCCTGGAGCATGG NGG 10 8 17 8 24 0 100 5 10
    240607023216_exce CCTGGTGTATGGGGTCGGGAATGGGGGTCCTG(+ATATAT)GATCCCTGCACTCCCCATGCTCCAGGCCCTG NGG 10 8 17 8 24 0 100 5 10
    240607023215_wrck CTTGACAGGGCCTGGAGCATGGGGAGTGCAGG(+ATATAT)GATCCAGGACCCCCATTCCCGACCCCATACA NGG 10 8 17 8 24 0 100 5 10