Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023657_qbyf CACACACCAAAGCTGTTTGCTCTTGGCACTTG(+ATATAT)GTACCCATGATCAACCTTCCCATCCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240607023655_nlfp AGGAGAGGGAGGGATGGGAAGGTTGATCATGG(+ATATAT)GTACCAAGTGCCAAGAGCAAACAGCTTTGGT NGG 10 8 17 8 24 0 100 5 10
    240607023654_4jke AGGAGCAATTCAGTCAAGGCCTTACCACATTG(+ATATAT)GTACCAAAGCATAGAACCCTTGTAATTTATA NGG 10 8 17 8 24 0 100 5 10
    240607023653_7wuy CCTGGTATAAATTACAAGGGTTCTATGCTTTG(+ATATAT)GTACCAATGTGGTAAGGCCTTGACTGAATTG NGG 10 8 17 8 24 0 100 5 10
    240607023652_obdl CCATTTAGGCTATAAAACTCGGCATGTAGAAG(+ATATAT)GTACCACCTACCATAGTTGTGGGAACGTTTC NGG 10 8 17 8 24 0 100 5 10
    240607023650_hbx3 ATTTAGAAACGTTCCCACAACTATGGTAGGTG(+ATATAT)GTACCTTCTACATGCCGAGTTTTATAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240607023648_zv6i GTGGATTCCTTGTCCTCTTCTGACCTCCTTGG(+ATATAT)GTACCTGCATGTATTTGTATATACCCGTCTA NGG 10 8 17 8 24 0 100 5 10
    240607023647_7euq GTTTCTAGACGGGTATATACAAATACATGCAG(+ATATAT)GTACCCAAGGAGGTCAGAAGAGGACAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607023645_umcu CCCCTTTAATTTTTTCCTGTTTTGAGTACTGG(+ATATAT)GTACCTAATTCTGCAACATAATGCAATATTT NGG 10 8 17 8 24 0 100 5 10
    240607023644_nnm0 TCCAGAAATATTGCATTATGTTGCAGAATTAG(+ATATAT)GTACCCAGTACTCAAAACAGGAAAAAATTAA NGG 10 8 17 8 24 0 100 5 10