Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023759_r8dr TCGACTTGCCCAGAAAGCATATGCCACAACAG(+ATATAT)GATCCTTCTGCACACGTTTATTGGGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240607023758_11kr AATCAAGCTCTCCCAATAAACGTGTGCAGAAG(+ATATAT)GATCCTGTTGTGGCATATGCTTTCTGGGCAA NGG 10 8 17 8 24 0 100 5 10
    240607023756_lc1h AGCACTCTTGGGAGACCTGCTCACTGTTGGAG(+ATATAT)GTACCTGGGTATGGAGAGCTGTGGCATAGGG NGG 10 8 17 8 24 0 100 5 10
    240607023754_qtat GCTGACCCTATGCCACAGCTCTCCATACCCAG(+ATATAT)GTACCTCCAACAGTGAGCAGGTCTCCCAAGA NGG 10 8 17 8 24 0 100 5 10
    240607023752_hvpr GCCAACTGCGGCTACATGTCCCAGGGTGGGTG(+ATATAT)GTACCAAAGTGGGGAGAAGAGGAGGGTGTAA NGG 10 8 17 8 24 0 100 5 10
    240607023751_y87k GCCCATTACACCCTCCTCTTCTCCCCACTTTG(+ATATAT)GTACCACCCACCCTGGGACATGTAGCCGCAG NGG 10 8 17 8 24 0 100 5 10
    240607023750_oyk6 TCCTGCCTGGCTACATGAAGTGGTCACTTGAG(+ATATAT)GATCCATTCCACCCACTACTAGAAGTCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607023749_isup TTTAGCTGAGACTTCTAGTAGTGGGTGGAATG(+ATATAT)GATCCTCAAGTGACCACTTCATGTAGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240607023747_6cgg ATTTCCTAAAACAATTAAGAAATTTTGCTATG(+ATATAT)GATCCAAGTTTCCTTACCCATAGATCTGACA NGG 10 8 17 8 24 0 100 5 10
    240607023746_4mu8 CATCATGTCAGATCTATGGGTAAGGAAACTTG(+ATATAT)GATCCATAGCAAAATTTCTTAATTGTTTTAG NGG 10 8 17 8 24 0 100 5 10