Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023829_po3i TGCTTTATTTCTCGCAGCTGGATTGAACCCAG(+ATATAT)GATCCTGCATGCGCCAGTCAAGGACTTGACA NGG 10 8 17 8 24 0 100 5 10
    240607023828_cohf TTCATTGTCAAGTCCTTGACTGGCGCATGCAG(+ATATAT)GATCCTGGGTTCAATCCAGCTGCGAGAAATA NGG 10 8 17 8 24 0 100 5 10
    240607023827_f3rj ATTGTAAGAGACCCTTCCTGGGTACTAGCCTG(+ATATAT)GTACCTGGCTACTGAGCAGTGGGCAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240607023825_8atj TTCTCTAGGCTTGCCCACTGCTCAGTAGCCAG(+ATATAT)GTACCAGGCTAGTACCCAGGAAGGGTCTCTT NGG 10 8 17 8 24 0 100 5 10
    240607023824_pbt6 AGGCTTGGGGAAGGCTGAGGCCCTGTTATTTG(+ATATAT)GATCCTGCCACCTCAGGGTCCCAGGCCCCTT NGG 10 8 17 8 24 0 100 5 10
    240607023822_izoj GCAATAAGGGGCCTGGGACCCTGAGGTGGCAG(+ATATAT)GATCCAAATAACAGGGCCTCAGCCTTCCCCA NGG 10 8 17 8 24 0 100 5 10
    240607023820_zhf6 TAGGAAGGTGATGCTTAATACTATGTGCCCAG(+ATATAT)GATCCCTCTGAAGTTTGCTGGAAGGCATGAT NGG 10 8 17 8 24 0 100 5 10
    240607023818_w2tp GGAGAATCATGCCTTCCAGCAAACTTCAGAGG(+ATATAT)GATCCTGGGCACATAGTATTAAGCATCACCT NGG 10 8 17 8 24 0 100 5 10
    240607023816_a7es ACAGACATCTGTCAGGGGACAGGGAGCTTGGG(+ATATAT)GTACCTGAACCAGGTCAGCTGATGGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607023815_it7g AGTCTCTGCTTCCATCAGCTGACCTGGTTCAG(+ATATAT)GTACCCCAAGCTCCCTGTCCCCTGACAGATG NGG 10 8 17 8 24 0 100 5 10