Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023709_5xew GGAGCAGGGACCTGATCTCCTATCTGTCCTGG(+ATATAT)GATCCATTTTCAGAGGGGCAGTTTTATTTGG NGG 10 8 17 8 24 0 100 5 10
    240607023708_szzn CCAGGCCAAATAAAACTGCCCCTCTGAAAATG(+ATATAT)GATCCCAGGACAGATAGGAGATCAGGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240607023707_lfz2 GTTAAGGAACTGGCCTTGGATTTTCTTTCTCG(+ATATAT)GTACCCATCTCTTTTTACTTACACTCTACAG NGG 10 8 17 8 24 0 100 5 10
    240607023705_xc9e AATATCTGTAGAGTGTAAGTAAAAAGAGATGG(+ATATAT)GTACCGAGAAAGAAAATCCAAGGCCAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240607023704_l9ug CGGGAGGACCTAGTTCTCCTCTGATCTCTGAG(+ATATAT)GTACCTGCACTTGTGCGTGCCAGACCCATGC NGG 10 8 17 8 24 0 100 5 10
    240607023703_17ky TCTGTGCATGGGTCTGGCACGCACAAGTGCAG(+ATATAT)GTACCTCAGAGATCAGAGGAGAACTAGGTCC NGG 10 8 17 8 24 0 100 5 10
    240607023701_fj67 GGGAATCTGATGCTCTCCTCAGACCTCTGAAG(+ATATAT)GTACCAGACATGTACTTAGTAAACAGACATG NGG 10 8 17 8 24 0 100 5 10
    240607023700_jnyp GCATGCATGTCTGTTTACTAAGTACATGTCTG(+ATATAT)GTACCTTCAGAGGTCTGAGGAGAGCATCAGA NGG 10 8 17 8 24 0 100 5 10
    240607023659_rwn9 GAGAGGTAGAATTGGCCACCTGGGGGGACGTG(+ATATAT)GTACCAGAAGTAGTCGGGATACCTTTAGAGT NGG 10 8 17 8 24 0 100 5 10
    240607023658_slgk CGGCTACTCTAAAGGTATCCCGACTACTTCTG(+ATATAT)GTACCACGTCCCCCCAGGTGGCCAATTCTAC NGG 10 8 17 8 24 0 100 5 10