Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023852_3at3 TACTCTAGGGGCTCTGAGGTCAATCCCCAATG(+ATATAT)GTACCTTCCCACAACAAGCCCCAAGTAATCA NGG 10 8 17 8 24 0 100 5 10
    240607023850_61xs ATGGTGGCTTCAGACTCTCTCCAGTTCCAGGG(+ATATAT)GATCCAATGCCCCAATACCCTCTCCAGCCAC NGG 10 8 17 8 24 0 100 5 10
    240607023849_de95 GGAGAGTGGCTGGAGAGGGTATTGGGGCATTG(+ATATAT)GATCCCCTGGAACTGGAGAGAGTCTGAAGCC NGG 10 8 17 8 24 0 100 5 10
    240607023847_l6e5 AGTGTAGGACTGGAGACTGAGAAGGGGTTTGG(+ATATAT)GTACCCGCGCTCTTCCGAGGAAGTGCTGTTA NGG 10 8 17 8 24 0 100 5 10
    240607023846_ye4u TCTCTTAACAGCACTTCCTCGGAAGAGCGCGG(+ATATAT)GTACCCAAACCCCTTCTCAGTCTCCAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240607023844_bris GCTTGCTCCGTTGCGCACTTTGGATCTCTGGG(+ATATAT)GATCCCTTTCTTCTTGGCTCACTGAGATGAT NGG 10 8 17 8 24 0 100 5 10
    240607023842_nys6 CCTACATCATCTCAGTGAGCCAAGAAGAAAGG(+ATATAT)GATCCCCAGAGATCCAAAGTGCGCAACGGAG NGG 10 8 17 8 24 0 100 5 10
    240607023841_6axn GGCTCAACGCTGGAATCAGTTTCCCTGAAAAG(+ATATAT)GATCCTCGGACTAAAAGTGGCATGTTAGAAA NGG 10 8 17 8 24 0 100 5 10
    240607023839_skal GGTTTTTTCTAACATGCCACTTTTAGTCCGAG(+ATATAT)GATCCTTTTCAGGGAAACTGATTCCAGCGTT NGG 10 8 17 8 24 0 100 5 10
    240607023838_h493 GAACGACAACCTGAGAGAAGCAGGTGGACTGG(+ATATAT)GTACCTTCTGCTCTCCACTGGCCATTGGCAG NGG 10 8 17 8 24 0 100 5 10