Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607023955_mx8o ATACAGTGCCTTCACCATGGTCACAAATTCTG(+ATATAT)GTACCTTTGTGCAAAAGTTGTGGTTGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240607023954_v854 AGCAAAAAACCAACCACAACTTTTGCACAAAG(+ATATAT)GTACCAGAATTTGTGACCATGGTGAAGGCAC NGG 10 8 17 8 24 0 100 5 10
    240607023953_m5ek CCTGGCTGGAGATGCTGCTGGGAGAGGCTGTG(+ATATAT)GTACCCGTAGGGAGAATCCCTAGCTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240607023951_zpu0 GTGGAGGTGGGAGCTAGGGATTCTCCCTACGG(+ATATAT)GTACCACAGCCTCTCCCAGCAGCATCTCCAG NGG 10 8 17 8 24 0 100 5 10
    240607023950_s27d TGGGAGCTGGCATCAACAGTATGGTCCTATTG(+ATATAT)GATCCTAGCAGACCTTTTTGCTACAGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240607023949_u53z GTATCCTCCACTGTAGCAAAAAGGTCTGCTAG(+ATATAT)GATCCAATAGGACCATACTGTTGATGCCAGC NGG 10 8 17 8 24 0 100 5 10
    240607023948_jc8u AGAGGCTTTCTGTGCTACACTGTCTTAGCTCG(+ATATAT)GATCCGGTGGGGGTTTGGCTGTCCTGTGGCC NGG 10 8 17 8 24 0 100 5 10
    240607023945_99qy GGGCAGGCCACAGGACAGCCAAACCCCCACCG(+ATATAT)GATCCGAGCTAAGACAGTGTAGCACAGAAAG NGG 10 8 17 8 24 0 100 5 10
    240607023944_ajnm CCTGGCTGGAGATGCTGCTGGGAGAGGCTGTG(+ATATAT)GTACCCGTAGGGAGAATCCCTAGCTCCCACC NGG 10 8 17 8 24 0 100 5 10
    240607023943_j8or GTGGAGGTGGGAGCTAGGGATTCTCCCTACGG(+ATATAT)GTACCACAGCCTCTCCCAGCAGCATCTCCAG NGG 10 8 17 8 24 0 100 5 10