Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024121_a6z5 CTTGCCACACAGCAAGGCCGATGGCATTTGTG(+ATATAT)GTACCTTGGGATGCCAGGGCCAGAGCATGGG NGG 10 8 17 8 24 0 100 5 10
    240607024119_4w1u CCTTTAAAGCTGTGTCCACCTGTCAGTACCAG(+ATATAT)GTACCATGGCAACGGCGGCAGCCACTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240607024117_ycgs GGTAGGGGGAAGTGGCTGCCGCCGTTGCCATG(+ATATAT)GTACCTGGTACTGACAGGTGGACACAGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607024116_7ri7 CGAGCTCCAGGGACTTGGTGTTGCTTGGGAGG(+ATATAT)GATCCATACAGCATTTTGCGTTCTTCATGTG NGG 10 8 17 8 24 0 100 5 10
    240607024115_1ekd GATTACACATGAAGAACGCAAAATGCTGTATG(+ATATAT)GATCCCTCCCAAGCAACACCAAGTCCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240607024113_ze57 CTCCTACTTTGTTCCCAGTAGTCCCGCGTAGG(+ATATAT)GATCCTGACCCGGCAGTGTGCTAAGCAAGAG NGG 10 8 17 8 24 0 100 5 10
    240607024112_yrnl CTGGCCTCTTGCTTAGCACACTGCCGGGTCAG(+ATATAT)GATCCCTACGCGGGACTACTGGGAACAAAGT NGG 10 8 17 8 24 0 100 5 10
    240607024111_2ubz CCGAGGGGGAAATTATGTCCACGGCCTCGCTG(+ATATAT)GTACCATGGAAGACTCATGCCCCCTGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240607024109_aqdj GGGAAATGGGCAGGGGGCATGAGTCTTCCATG(+ATATAT)GTACCAGCGAGGCCGTGGACATAATTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240607024108_95qf GCACATGGATCTCACCCGTATTCTGTTGTATG(+ATATAT)GTACCTCGGTACATCTTTGTGGTTGTGTTAA NGG 10 8 17 8 24 0 100 5 10