Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607024536_zluh GTAGCCTTTGCGATTGTGTGATCCAAGAGCTG(+ATATAT)GATCCATACAATTTGTGCCACCCTGAAATTG NGG 10 8 17 8 24 0 100 5 10
    240607024534_6tjr TCAACCCTGAGGTGTCTGTCCACTATCTGAAG(+ATATAT)GATCCAATTATTTCTAGCTCCCATCCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240607024533_1y8b AGGGTAGGAGGGATGGGAGCTAGAAATAATTG(+ATATAT)GATCCTTCAGATAGTGGACAGACACCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240607024532_wurb TCATCTGGGAAAGCACTGATTACACTTTACAG(+ATATAT)GTACCTGTCAGCCCTGAGCTTCACAGACAAA NGG 10 8 17 8 24 0 100 5 10
    240607024531_s6eo TCGTGTTTGTCTGTGAAGCTCAGGGCTGACAG(+ATATAT)GTACCTGTAAAGTGTAATCAGTGCTTTCCCA NGG 10 8 17 8 24 0 100 5 10
    240607024529_gpw2 ACTCAATGCAAACCTAACTGGCTATTAGCTCG(+ATATAT)GATCCTTTGAATCTTTGAGTTACAAAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240607024528_ezdq TGAAAGAGCTTTTGTAACTCAAAGATTCAAAG(+ATATAT)GATCCGAGCTAATAGCCAGTTAGGTTTGCAT NGG 10 8 17 8 24 0 100 5 10
    240607024527_p7cd CAAGGTCCACTCAACTATTCTGGCTTCTGTGG(+ATATAT)GTACCAGGCACACATGTGTAGTACATCCACA NGG 10 8 17 8 24 0 100 5 10
    240607024525_ffks GCATGTGTGGATGTACTACACATGTGTGCCTG(+ATATAT)GTACCCACAGAAGCCAGAATAGTTGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240607024524_e7yt CTTTGTCGAAAGCTTTCATGTCAAAAGTAAAG(+ATATAT)GTACCCATCGTGACCAGGATTGCTTAACTAT NGG 10 8 17 8 24 0 100 5 10