Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022255_v624 AATAGGCCAGACCATTCTACACTTTTCCACTG(+ATATAT)GATCCTTTCCTTATTTTTTTTGGATCATAAT NGG 10 8 17 8 24 0 100 5 10
    240607022253_jjvz AACGAATTATGATCCAAAAAAAATAAGGAAAG(+ATATAT)GATCCAGTGGAAAAGTGTAGAATGGTCTGGC NGG 10 8 17 8 24 0 100 5 10
    240607022252_uiid AGAAAGGCTTAAGAAACCCCCAGTCCCTTAAG(+ATATAT)GTACCTAGTTTCTTACTACTACAGTGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240607022251_6cg4 CATGGGAGCCCACTGTAGTAGTAAGAAACTAG(+ATATAT)GTACCTTAAGGGACTGGGGGTTTCTTAAGCC NGG 10 8 17 8 24 0 100 5 10
    240607022249_23cd TAGTCCCCAGGAATGAAACAATGATATGCCTG(+ATATAT)GATCCTGCTGACCTTCGCCACCTCAACCAAT NGG 10 8 17 8 24 0 100 5 10
    240607022248_772z AATCAATTGGTTGAGGTGGCGAAGGTCAGCAG(+ATATAT)GATCCAGGCATATCATTGTTTCATTCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240607022247_hjxf GGTGTCTTTATCAAAGGTATATATAGAATTTG(+ATATAT)GTACCTCTCAGGTTGTATCTCTAAAGGTTAC NGG 10 8 17 8 24 0 100 5 10
    240607022246_umcu TTCCTGTAACCTTTAGAGATACAACCTGAGAG(+ATATAT)GTACCAAATTCTATATATACCTTTGATAAAG NGG 10 8 17 8 24 0 100 5 10
    240607022244_k9oj CCTACACATTAAATGTTTCTTTCAGGCTGGGG(+ATATAT)GTACCGCAAGGTGGCAGAACTTCACCTAGCA NGG 10 8 17 8 24 0 100 5 10
    240607022243_tilw CTATGTGCTAGGTGAAGTTCTGCCACCTTGCG(+ATATAT)GTACCCCCAGCCTGAAAGAAACATTTAATGT NGG 10 8 17 8 24 0 100 5 10