Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022211_kua7 GGGCGTCCGGGGACAGAAGGAGCTGTCTACTG(+ATATAT)GTACCTCCTCTCTATAGGCACTCTGGTCCGT NGG 10 8 17 8 24 0 100 5 10
    240607022209_jk4h AGGGGACGGACCAGAGTGCCTATAGAGAGGAG(+ATATAT)GTACCAGTAGACAGCTCCTTCTGTCCCCGGA NGG 10 8 17 8 24 0 100 5 10
    240607022208_ytrs TCCTAAAATATTTTTAAAAGGGAAGATTAATG(+ATATAT)GTACCAAGAACTAAGATCTAGGTAGATGAGA NGG 10 8 17 8 24 0 100 5 10
    240607022207_g9xu AACCATCTCATCTACCTAGATCTTAGTTCTTG(+ATATAT)GTACCATTAATCTTCCCTTTTAAAAATATTT NGG 10 8 17 8 24 0 100 5 10
    240607022206_9y1l GGGGATCCAACACCTTCTTCTGGCCTCTGTAG(+ATATAT)GTACCAGGCATGCAAAAAACAAAACAAAACA NGG 10 8 17 8 24 0 100 5 10
    240607022204_gwq6 TGTTTTGTTTTGTTTTGTTTTTTGCATGCCTG(+ATATAT)GTACCTACAGAGGCCAGAAGAAGGTGTTGGA NGG 10 8 17 8 24 0 100 5 10
    240607022202_25qi TTGCCACTCTTGCAACTCCTATCACCCTCATG(+ATATAT)GTACCTTATAACCATTTATAATTCCAGTTCG NGG 10 8 17 8 24 0 100 5 10
    240607022201_ikv6 TTCCTCGAACTGGAATTATAAATGGTTATAAG(+ATATAT)GTACCATGAGGGTGATAGGAGTTGCAAGAGT NGG 10 8 17 8 24 0 100 5 10
    240607022200_cc02 AAGTGCCCCTAGCTGCTTCTCTCTGGTCCAAG(+ATATAT)GTACCTGATTCTAGTAAGGAGAGCACTGAGC NGG 10 8 17 8 24 0 100 5 10
    240607022159_acr5 ATTGTGCTCAGTGCTCTCCTTACTAGAATCAG(+ATATAT)GTACCTTGGACCAGAGAGAAGCAGCTAGGGG NGG 10 8 17 8 24 0 100 5 10