Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022124_kdzt CAAACTTTGTGGCTTAGAAGCCTGAAGAAGGG(+ATATAT)GATCCCTTCCCTCCGAGAGCGCTTAACCCTG NGG 10 8 17 8 24 0 100 5 10
    240607022122_gj4e CATCTCAGGGTTAAGCGCTCTCGGAGGGAAGG(+ATATAT)GATCCCCTTCTTCAGGCTTCTAAGCCACAAA NGG 10 8 17 8 24 0 100 5 10
    240607022121_n3qt GACACCCCTGCCTTAGCAAGGGGCTCCAGTTG(+ATATAT)GATCCCGTTGAAGCACGGTGGCCGCGAAGGT NGG 10 8 17 8 24 0 100 5 10
    240607022119_pwbi GCCCTACCTTCGCGGCCACCGTGCTTCAACGG(+ATATAT)GATCCAACTGGAGCCCCTTGCTAAGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607022118_1c6a GGAACAGGGCAGGAGGAGAGAGAGAGTTTGTG(+ATATAT)GATCCCCAGAAACTCTAGGCCCATATAGTCG NGG 10 8 17 8 24 0 100 5 10
    240607022116_vhid TTGGTCGACTATATGGGCCTAGAGTTTCTGGG(+ATATAT)GATCCACAAACTCTCTCTCTCCTCCTGCCCT NGG 10 8 17 8 24 0 100 5 10
    240607022114_ccfn ACCACATGATAGTTCACAACCATCTGCAATGG(+ATATAT)GATCCAATGCCTTCTTCTGGTCTGTCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240607022113_i1cr ACTGTCTTCTGACAGACCAGAAGAAGGCATTG(+ATATAT)GATCCCATTGCAGATGGTTGTGAACTATCAT NGG 10 8 17 8 24 0 100 5 10
    240607022112_ffft TGCTTCAAGGATAGGAGAGCCTCAGGTGAGAG(+ATATAT)GTACCAACCACAGTGTGGGTGACTCGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240607022111_m0gy CCAGAAGGGCCGAGTCACCCACACTGTGGTTG(+ATATAT)GTACCTCTCACCTGAGGCTCTCCTATCCTTG NGG 10 8 17 8 24 0 100 5 10