Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022055_l3x6 TACTTAAAACTGCATACCAGAAGAGGGCATCG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607022054_h7va TACATCCCCAGCCAGCCTTTGTCTTTCTGTGG(+ATATAT)GTACCTGTGGTGCTGGGACCCGAACCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607022052_4kir TATTCCTGAGGGTTCGGGTCCCAGCACCACAG(+ATATAT)GTACCCACAGAAAGACAAAGGCTGGCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240607022050_l81o ACCACATGGTGGCTCACAACCATCTGTAATGG(+ATATAT)GATCCTCTTCTGGTGTGTCTGAAGACAGTGA NGG 10 8 17 8 24 0 100 5 10
    240607022049_5eyv CACCGTCACTGTCTTCAGACACACCAGAAGAG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240607022048_8ys6 TTGACCTCTTCGTCCTTTTGTTTTTAGATCTG(+ATATAT)GTACCACTGGGATGATGACCTGAGACCCCCA NGG 10 8 17 8 24 0 100 5 10
    240607022046_ziws TCCAGTGGGGGTCTCAGGTCATCATCCCAGTG(+ATATAT)GTACCAGATCTAAAAACAAAAGGACGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240607022045_vgmm CAGTCTGAGAATGAGTGTGTGTGAGTGAGGCG(+ATATAT)GTACCCACATTCTCAACTTCAAGTCATTGCA NGG 10 8 17 8 24 0 100 5 10
    240607022044_9ekt GAAACTGCAATGACTTGAAGTTGAGAATGTGG(+ATATAT)GTACCGCCTCACTCACACACACTCATTCTCA NGG 10 8 17 8 24 0 100 5 10
    240607022042_z3mo CTCTTCCACTTTGTAAGAGGATTTTTCTGCTG(+ATATAT)GATCCCTCCTCTATGCCATCCAGCTCTGACC NGG 10 8 17 8 24 0 100 5 10