Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021957_ol5m AGTTTTCCACTTCACCATGGCCTCCTCCCCTG(+ATATAT)GATCCAGGCCCCAACCCCCACCTTTCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240607021954_0zfm GCTTTGATACCTGGTTCAAGTGACCATGGGGG(+ATATAT)GATCCCATAGTACCTGGAATGAGATTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607021952_tmkm ACGCTCCAGCAATCTCATTCCAGGTACTATGG(+ATATAT)GATCCCCCCATGGTCACTTGAACCAGGTATC NGG 10 8 17 8 24 0 100 5 10
    240607021951_pzdc GGGGGGAGGGGGCGCGCTCTGAGATAGAGCAG(+ATATAT)GATCCTTCTGCCCTGGGGCCTGGTCCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240607021950_e30w CTGGTAGACTGGACCAGGCCCCAGGGCAGAAG(+ATATAT)GATCCTGCTCTATCTCAGAGCGCGCCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240607021948_ku8y ACAAAAAAAATAGTAGGATTGGACATTGGAGG(+ATATAT)GTACCAAAAGCAGACTCCTAACAAGGGGAAC NGG 10 8 17 8 24 0 100 5 10
    240607021947_bacv TCCCTGTTCCCCTTGTTAGGAGTCTGCTTTTG(+ATATAT)GTACCCTCCAATGTCCAATCCTACTATTTTT NGG 10 8 17 8 24 0 100 5 10
    240607021945_3h15 CGAGGAATCTGGTACTGGCATGCGGATTGGGG(+ATATAT)GATCCTGAGTCCGTTTGAAAGACTGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240607021944_f8kb CCTCCTCTGCCCAGTCTTTCAAACGGACTCAG(+ATATAT)GATCCCCCAATCCGCATGCCAGTACCAGATT NGG 10 8 17 8 24 0 100 5 10
    240607021943_1lbv TTCCTTGGCTTCGGTTTCTGCTGCTCTCATAG(+ATATAT)GATCCCTTAAGAGAGGGGATGACTCGTCAAG NGG 10 8 17 8 24 0 100 5 10