Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021941_xt0k TGATCCTTGACGAGTCATCCCCTCTCTTAAGG(+ATATAT)GATCCTATGAGAGCAGCAGAAACCGAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240607021939_xkgt CGAGGAATCTGGTACTGGCATGCGGATTGGGG(+ATATAT)GATCCTGAGTCCGTTTGAAAGACTGGGCAGA NGG 10 8 17 8 24 0 100 5 10
    240607021938_so03 CCTCCTCTGCCCAGTCTTTCAAACGGACTCAG(+ATATAT)GATCCCCCAATCCGCATGCCAGTACCAGATT NGG 10 8 17 8 24 0 100 5 10
    240607021936_uhoh TCAAATGACTGCAGCCCCAAGCACAGCCCTAG(+ATATAT)GATCCAAACCAGGAAGAAAACAAATATTATT NGG 10 8 17 8 24 0 100 5 10
    240607021934_ugna CCAGGAATAATATTTGTTTTCTTCCTGGTTTG(+ATATAT)GATCCTAGGGCTGTGCTTGGGGCTGCAGTCA NGG 10 8 17 8 24 0 100 5 10
    240607021933_zyay TTGGGGGATGCCTGGGCCAGGGAGAGGCACTG(+ATATAT)GTACCAGCCGTCCAAGCTGACAGCCTTACCC NGG 10 8 17 8 24 0 100 5 10
    240607021932_dljt CTTAGGGGTAAGGCTGTCAGCTTGGACGGCTG(+ATATAT)GTACCAGTGCCTCTCCCTGGCCCAGGCATCC NGG 10 8 17 8 24 0 100 5 10
    240607021930_oek8 GCACTGAGTTCTAGGCCTCCTCTTGTCTCCTG(+ATATAT)GATCCTCACTTGAATCCTGCAGCAGAACGGA NGG 10 8 17 8 24 0 100 5 10
    240607021929_4zgt GGTTGTCCGTTCTGCTGCAGGATTCAAGTGAG(+ATATAT)GATCCAGGAGACAAGAGGAGGCCTAGAACTC NGG 10 8 17 8 24 0 100 5 10
    240607021927_cb4s AAGTGGCAAGAGATGGATTCTGAAAGGGGAGG(+ATATAT)GTACCTGAGGGGGTCTCACCTCCCTTGGTCC NGG 10 8 17 8 24 0 100 5 10