Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607021856_7ieb TAATGTTAAGCCCAGGCTGGCCTTGAACTCAG(+ATATAT)GATCCTCTTACCTCCACCCCCTTAAGTGCTG NGG 10 8 17 8 24 0 100 5 10
    240607021854_lj3u GAGGCTAATACCAAACCTATAGCTACTCCTGG(+ATATAT)GTACCCTGCCTCCAGGTGATCACAGCCATCC NGG 10 8 17 8 24 0 100 5 10
    240607021852_32ki CGCAAGGATGGCTGTGATCACCTGGAGGCAGG(+ATATAT)GTACCCAGGAGTAGCTATAGGTTTGGTATTA NGG 10 8 17 8 24 0 100 5 10
    240607021850_up8o AGAGCACCCCATCCAACAGGCTTCTTGCCCTG(+ATATAT)GTACCATGGAAGCACAGTTCTGGGTTTCATG NGG 10 8 17 8 24 0 100 5 10
    240607021848_6toq CTCAGCATGAAACCCAGAACTGTGCTTCCATG(+ATATAT)GTACCAGGGCAAGAAGCCTGTTGGATGGGGT NGG 10 8 17 8 24 0 100 5 10
    240607021847_8d0d CTAACTCTAAAATCCAAAAAATCTCCCAACAG(+ATATAT)GTACCAAGAAGGTTCATTTGTAATGACAGTT NGG 10 8 17 8 24 0 100 5 10
    240607021846_odz4 AAATGAACTGTCATTACAAATGAACCTTCTTG(+ATATAT)GTACCTGTTGGGAGATTTTTTGGATTTTAGA NGG 10 8 17 8 24 0 100 5 10
    240607021845_iwwv CTGGTATTTCAGCAACAAGTACCTTCTTGAAG(+ATATAT)GTACCCAAAGTGATTCCAATTTTATGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240607021843_g22c TCAGCTTTTTCATAAAATTGGAATCACTTTGG(+ATATAT)GTACCTTCAAGAAGGTACTTGTTGCTGAAAT NGG 10 8 17 8 24 0 100 5 10
    240607021841_6nva AAAGCTGAGGTTTAGAAAGAAGTGGTCCCAAG(+ATATAT)GATCCCACCATGCCTTTGTGTTGATGCTGGA NGG 10 8 17 8 24 0 100 5 10