Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607022241_x5ak GTTCCTTTTCTCGGTTTGCTGAGGGCCTCTTG(+ATATAT)GATCCACTGGCCCAGAGATTTAAAAGTTTGG NGG 10 8 17 8 24 0 100 5 10
    240607022239_rif4 CAACCCCAAACTTTTAAATCTCTGGGCCAGTG(+ATATAT)GATCCAAGAGGCCCTCAGCAAACCGAGAAAA NGG 10 8 17 8 24 0 100 5 10
    240607022238_9gp4 AGTATATCTCTGCCCCAGCTCCAGTCTTGCTG(+ATATAT)GATCCTCCTCCAGCTCCAACACACACACACA NGG 10 8 17 8 24 0 100 5 10
    240607022237_0upj GTGTGTGTGTGTGTGTGTTGGAGCTGGAGGAG(+ATATAT)GATCCAGCAAGACTGGAGCTGGGGCAGAGAT NGG 10 8 17 8 24 0 100 5 10
    240607022235_iaij CGACTCGGCGGTGAACGAACCTCAGGGTGCGG(+ATATAT)GATCCTACACACACTCCCTTTCCCCACCCCC NGG 10 8 17 8 24 0 100 5 10
    240607022234_63lj GGGTGGGGGGTGGGGAAAGGGAGTGTGTGTAG(+ATATAT)GATCCCGCACCCTGAGGTTCGTTCACCGCCG NGG 10 8 17 8 24 0 100 5 10
    240607022233_ft8b CACTGCCTTCTCCTTCCTGTCCCCCCTGGAAG(+ATATAT)GTACCCAGTTCTGTCTGAGAGGACAAGCGGG NGG 10 8 17 8 24 0 100 5 10
    240607022231_5gby CCGGGCCCGCTTGTCCTCTCAGACAGAACTGG(+ATATAT)GTACCTTCCAGGGGGGACAGGAAGGAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240607022230_ro66 CGGAGGCGGGGAGGCCGCTCCTCTTTGGGCAG(+ATATAT)GATCCTACTATGGACACAGAACAGACAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607022228_vq8x GGCAGCTGCTGTCTGTTCTGTGTCCATAGTAG(+ATATAT)GATCCTGCCCAAAGAGGAGCGGCCTCCCCGC NGG 10 8 17 8 24 0 100 5 10