Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015801_5o1v TGGAGATGAGCTGCTTATGAAGTCTGGTACAG(+ATATAT)GTACCCATGTCTTCGGGTGGTGGTGCCAACA NGG 10 8 17 8 24 0 100 5 10
    240607015800_8fks CACCAGGCTAGGCTCATGGCCTTGAACTCAGG(+ATATAT)GATCCACCTGCCTCTGCCTCCCTGTGATGCT NGG 10 8 17 8 24 0 100 5 10
    240607015759_0qte ATCTCAGCATCACAGGGAGGCAGAGGCAGGTG(+ATATAT)GATCCCTGAGTTCAAGGCCATGAGCCTAGCC NGG 10 8 17 8 24 0 100 5 10
    240607015757_s01q GCCTGAAGAGGGCCTGGGGAACAGTGGAAATG(+ATATAT)GTACCTTCATGGTGACTTCAGCAGGGTAGTC NGG 10 8 17 8 24 0 100 5 10
    240607015756_t2w7 GTAATGACTACCCTGCTGAAGTCACCATGAAG(+ATATAT)GTACCATTTCCACTGTTCCCCAGGCCCTCTT NGG 10 8 17 8 24 0 100 5 10
    240607015755_ji7d ATAGCTGTGGTATCAGGACTTGAGAGATGGAG(+ATATAT)GATCCAAAAGTTTAGGGTCAGTCTGGGTATA NGG 10 8 17 8 24 0 100 5 10
    240607015753_2bmw TGCTGTATACCCAGACTGACCCTAAACTTTTG(+ATATAT)GATCCTCCATCTCTCAAGTCCTGATACCACA NGG 10 8 17 8 24 0 100 5 10
    240607015751_hjj0 TCCTGCCAGCCCTTGAGCCTCTGCTCTCCTGG(+ATATAT)GTACCCTCCTGGAACACCATGCTAACCTTCC NGG 10 8 17 8 24 0 100 5 10
    240607015749_bnk9 CTCGGGGAAGGTTAGCATGGTGTTCCAGGAGG(+ATATAT)GTACCCAGGAGAGCAGAGGCTCAAGGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607015748_8trc GTGTCCTGTTTGATGTCTGAGGTCTGCTTTGG(+ATATAT)GTACCGCCCTGGGAACTGCTAACCTCCGATT NGG 10 8 17 8 24 0 100 5 10