Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015729_4l7m CTTGCCTGCCTAAAAGAATGCAGCACTAACTG(+ATATAT)GATCCTGGGGGCTCCTGCACAAGCCCCTCCT NGG 10 8 17 8 24 0 100 5 10
    240607015727_hcsp AAGAAAATGACATGGCCTGGAGGAGATTCGGG(+ATATAT)GTACCTTTCCTCAGTGAGCTTTGATGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240607015726_8ehx TTTTGGACCACATCAAAGCTCACTGAGGAAAG(+ATATAT)GTACCCCGAATCTCCTCCAGGCCATGTCATT NGG 10 8 17 8 24 0 100 5 10
    240607015724_sulh GTTAACTGAGGGGCTAGATGTTGGCCCATGTG(+ATATAT)GATCCCAACTTTAACATGACCAAGATTGGGT NGG 10 8 17 8 24 0 100 5 10
    240607015723_i3o5 GCCCTACCCAATCTTGGTCATGTTAAAGTTGG(+ATATAT)GATCCACATGGGCCAACATCTAGCCCCTCAG NGG 10 8 17 8 24 0 100 5 10
    240607015720_wubc AGAGAAGACACATTCCATCATTCCACCCCAGG(+ATATAT)GTACCCTTCCACCCACAAAACTTATAAAATA NGG 10 8 17 8 24 0 100 5 10
    240607015718_8rxe TATTTTATTTTATAAGTTTTGTGGGTGGAAGG(+ATATAT)GTACCCTGGGGTGGAATGATGGAATGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607015717_cd5d TAAAATAAAATAAAATAAAAGGATTCCTTCTG(+ATATAT)GATCCCAGTCCCCTTAGCTCTCTCACATCCC NGG 10 8 17 8 24 0 100 5 10
    240607015715_xzfo TGGTTGGGATGTGAGAGAGCTAAGGGGACTGG(+ATATAT)GATCCAGAAGGAATCCTTTTATTTTATTTTA NGG 10 8 17 8 24 0 100 5 10
    240607015713_k6sl CCAGCACATTTTGAGCCCCCTCCCCACCTCCG(+ATATAT)GATCCTGCTCCTGAGGTCCCTCAGTCCATGG NGG 10 8 17 8 24 0 100 5 10