Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015641_smhv CCTCTACTACTTAGGGCTATGCTACACTGAGG(+ATATAT)GATCCTACCAATTCTCTCTAGAACAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240607015640_4i49 TCTGTCATAATGTAGATCATGGTAGGTATGTG(+ATATAT)GATCCCCTAAACATCTTCACATAGTGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240607015638_n0qw CATTGGAGCACACTATGTGAAGATGTTTAGGG(+ATATAT)GATCCACATACCTACCATGATCTACATTATG NGG 10 8 17 8 24 0 100 5 10
    240607015637_xfrf TCTATTCCTGCCGAAGCTTGCCAGGGCAGGTG(+ATATAT)GTACCCAAGAGGCTCTTCCTTTCCTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607015635_bz6h CTTCTCTTCAGAGGAAAGGAAGAGCCTCTTGG(+ATATAT)GTACCACCTGCCCTGGCAAGCTTCGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240607015634_0ysk CTGTATTAAGCAGAACCCAGGTCGTTTGCTTG(+ATATAT)GATCCACGATTTGACTTCAAGTTGTTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607015633_wtpj TTCGAAGACAAACAACTTGAAGTCAAATCGTG(+ATATAT)GATCCAAGCAAACGACCTGGGTTCTGCTTAA NGG 10 8 17 8 24 0 100 5 10
    240607015632_0t19 TTACAGACTGGGTGGTAAAGAGGGGCATCTTG(+ATATAT)GTACCTGGGTCCCAGAGAGAGAAGTCAAACA NGG 10 8 17 8 24 0 100 5 10
    240607015629_xudz CATTTTGTTTGACTTCTCTCTCTGGGACCCAG(+ATATAT)GTACCAAGATGCCCCTCTTTACCACCCAGTC NGG 10 8 17 8 24 0 100 5 10
    240607015628_ugzy TAGGGCTCACTTGTATATTAGTTTCTGAATTG(+ATATAT)GATCCATAACTTTAAGATTAAACAGAGAAAA NGG 10 8 17 8 24 0 100 5 10