Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015615_rrld AACAAAAATGGCTAAAAATTTTTACAAATGAG(+ATATAT)GATCCATTCAATAGATTAAAAAAATCTCTTC NGG 10 8 17 8 24 0 100 5 10
    240607015612_y20z CCCCTGTCCTGCTGATGCTGTCTATCCCCTGG(+ATATAT)GATCCAGGGTCCTCTGCCTGCCCTCCTTTGG NGG 10 8 17 8 24 0 100 5 10
    240607015610_264x CTTTTCCAAAGGAGGGCAGGCAGAGGACCCTG(+ATATAT)GATCCCAGGGGATAGACAGCATCAGCAGGAC NGG 10 8 17 8 24 0 100 5 10
    240607015609_aj6f TTCCTGACGGCTGATGTGGGCCAGTGGCAGAG(+ATATAT)GATCCAGGTTGCCCTTCCTTCCCCCCACCAC NGG 10 8 17 8 24 0 100 5 10
    240607015607_ogok TTCCTGTGGTGGGGGGAAGGAAGGGCAACCTG(+ATATAT)GATCCTCTGCCACTGGCCCACATCAGCCGTC NGG 10 8 17 8 24 0 100 5 10
    240607015605_w04s TGGGACACTTGGCAGAAGGGATTGCCCTGGGG(+ATATAT)GTACCAAGAGCCTAGGGTGCTCCCTGTAGGT NGG 10 8 17 8 24 0 100 5 10
    240607015604_71gk CTGGGACCTACAGGGAGCACCCTAGGCTCTTG(+ATATAT)GTACCCCCAGGGCAATCCCTTCTGCCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240607015602_ub3o CCTGCGTCTCCCAGCATGGTAGGTAGCCTCAG(+ATATAT)GATCCTAGAAAGATTATGGCCAGTGTCTGTT NGG 10 8 17 8 24 0 100 5 10
    240607015601_fejw GTCAGAACAGACACTGGCCATAATCTTTCTAG(+ATATAT)GATCCTGAGGCTACCTACCATGCTGGGAGAC NGG 10 8 17 8 24 0 100 5 10
    240607015559_59kr CAAGCAACTGGTAGCTCTCACAATTCTGTTGG(+ATATAT)GATCCTTTGTGCAATATTGTAGCCATCTTCC NGG 10 8 17 8 24 0 100 5 10