Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015530_8mgz TTTTTCCCTCACTGGAGGGGAAGGGGGCCATG(+ATATAT)GATCCTAAGCCATAAAATAAATTTTATTCCA NGG 10 8 17 8 24 0 100 5 10
    240607015529_6b4d TATCAGCCAGTGTGGCCCTCTATTTCCTTGTG(+ATATAT)GTACCGAGTGTCCCTGAGGCTGGGGTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607015528_dp04 GAAAAAGACCACCCCAGCCTCAGGGACACTCG(+ATATAT)GTACCACAAGGAAATAGAGGGCCACACTGGC NGG 10 8 17 8 24 0 100 5 10
    240607015526_r45w AGCATGCAGACATCCCTCTGTGGACTTCAGGG(+ATATAT)GATCCACCTCTAAAGAGAGTCACTAGTGACC NGG 10 8 17 8 24 0 100 5 10
    240607015525_tdff CTGTTGGTCACTAGTGACTCTCTTTAGAGGTG(+ATATAT)GATCCCCTGAAGTCCACAGAGGGATGTCTGC NGG 10 8 17 8 24 0 100 5 10
    240607015523_1qzz GGGTTCCCACGTGTCTGGAAGCTCGAGTTCCG(+ATATAT)GATCCATACTGACACCCAATAAGCTGTGTAG NGG 10 8 17 8 24 0 100 5 10
    240607015522_auqg TACTGCTACACAGCTTATTGGGTGTCAGTATG(+ATATAT)GATCCGGAACTCGAGCTTCCAGACACGTGGG NGG 10 8 17 8 24 0 100 5 10
    240607015520_opr7 AGACTCGGCCAGAGACAGACGTTAGAGGACGG(+ATATAT)GATCCAGTCCCTCTCCCACAGCAGGCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240607015519_1y9r TAAGTGCTTTGCCTGCTGTGGGAGAGGGACTG(+ATATAT)GATCCCGTCCTCTAACGTCTGTCTCTGGCCG NGG 10 8 17 8 24 0 100 5 10
    240607015518_cu5e GCAGAACCTAGGGCTTTGACCATGGCTTAGTG(+ATATAT)GTACCATGCGAAGACACTTAGTGTGACAAGT NGG 10 8 17 8 24 0 100 5 10