Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015517_yxp0 CTCAGACTTGTCACACTAAGTGTCTTCGCATG(+ATATAT)GTACCACTAAGCCATGGTCAAAGCCCTAGGT NGG 10 8 17 8 24 0 100 5 10
    240607015515_ticm ATCTGCCCATTTCTGTCCCCTGGGGTACTGGG(+ATATAT)GATCCAAACATAGTCCTCATGTCTGTTCAGT NGG 10 8 17 8 24 0 100 5 10
    240607015513_s5jj TGCTTACTGAACAGACATGAGGACTATGTTTG(+ATATAT)GATCCCCAGTACCCCAGGGGACAGAAATGGG NGG 10 8 17 8 24 0 100 5 10
    240607015512_0f1s AGTGTGCTTGCCTAGCATGTACAAAGCTCTGG(+ATATAT)GATCCACCCCCAGCACCACTTAAGCCAGGCG NGG 10 8 17 8 24 0 100 5 10
    240607015510_gg4d CGCCACGCCTGGCTTAAGTGGTGCTGGGGGTG(+ATATAT)GATCCCAGAGCTTTGTACATGCTAGGCAAGC NGG 10 8 17 8 24 0 100 5 10
    240607015509_bgzi CTTTGCCAAGGCCCCCCGCTGTCCTCTACTAG(+ATATAT)GATCCAACGGAAGCCTCTGCTCCCCCCGCCT NGG 10 8 17 8 24 0 100 5 10
    240607015507_vfe2 GAGAGAGGCGGGGGGAGCAGAGGCTTCCGTTG(+ATATAT)GATCCTAGTAGAGGACAGCGGGGGGCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240607015506_f2ip TGAAAGGACTCTTTTCTGCCCAAGGGACACAG(+ATATAT)GATCCGTCAGTTCCACTCCCTGCATGCACCA NGG 10 8 17 8 24 0 100 5 10
    240607015505_54us GGTCGTGGTGCATGCAGGGAGTGGAACTGACG(+ATATAT)GATCCTGTGTCCCTTGGGCAGAAAAGAGTCC NGG 10 8 17 8 24 0 100 5 10
    240607015502_nqop TTGGCCACTTGACTTTGGTCCAGTGATCCCGG(+ATATAT)GTACCGGTAACGGGCATAAGTTTCAGCTCCT NGG 10 8 17 8 24 0 100 5 10