Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015415_ivdi CATGTCAGTTTATGAACATTCTCATTACAATG(+ATATAT)GATCCTATAGTAAATGGCCCTAGTGACTATA NGG 10 8 17 8 24 0 100 5 10
    240607015414_o7d3 GCAGTTCCCACCTGTGGCCATGCTCAGCACTG(+ATATAT)GTACCCAGCCGATTTTCAGTCAGGCTTTCTG NGG 10 8 17 8 24 0 100 5 10
    240607015413_87pj AAAGGCAGAAAGCCTGACTGAAAATCGGCTGG(+ATATAT)GTACCAGTGCTGAGCATGGCCACAGGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240607015411_jr4q CCTTGGGTTTCATTTAGCTTCTCACAGTCAAG(+ATATAT)GATCCAAGACGCCAAATCTATGTGTGAGCAT NGG 10 8 17 8 24 0 100 5 10
    240607015410_oiwz GTCAAATGCTCACACATAGATTTGGCGTCTTG(+ATATAT)GATCCTTGACTGTGAGAAGCTAAATGAAACC NGG 10 8 17 8 24 0 100 5 10
    240607015409_4610 GTATTTAGAATGTTATATTTATTGTCGGTTTG(+ATATAT)GTACCACCTTGTCCCAGGTGAGCATTTGGCC NGG 10 8 17 8 24 0 100 5 10
    240607015408_wwhi CCATTGGCCAAATGCTCACCTGGGACAAGGTG(+ATATAT)GTACCAAACCGACAATAAATATAACATTCTA NGG 10 8 17 8 24 0 100 5 10
    240607015406_q04o AAAGAGGGGGAGGAATGAGACAGGACAGGCAG(+ATATAT)GATCCCTGAAATAACTGGAACGCACAATAAG NGG 10 8 17 8 24 0 100 5 10
    240607015404_auit GCCATCTTATTGTGCGTTCCAGTTATTTCAGG(+ATATAT)GATCCTGCCTGTCCTGTCTCATTCCTCCCCC NGG 10 8 17 8 24 0 100 5 10
    240607015403_z73h GAGTGTCAGGACTCTGAAGGAAGGGCAGCCAG(+ATATAT)GTACCAAGCTACCTCCTTCCCACCCTCAACC NGG 10 8 17 8 24 0 100 5 10