Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015500_qo8j GACCCAGGAGCTGAAACTTATGCCCGTTACCG(+ATATAT)GTACCCGGGATCACTGGACCAAAGTCAAGTG NGG 10 8 17 8 24 0 100 5 10
    240607015459_6e8y GGGGTATGTGGGAGCCGGTACACACGGTGCTG(+ATATAT)GATCCAGAGCTGGGGTGGGGTGCATGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240607015458_gqd1 ACCCCAGGGACATGCACCCCACCCCAGCTCTG(+ATATAT)GATCCAGCACCGTGTGTACCGGCTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240607015457_uhbx GGGGTATGTGGGAGCCGGTACACACGGTGCTG(+ATATAT)GATCCAGAGCTGGGGTGGGGTGCATGTCCCT NGG 10 8 17 8 24 0 100 5 10
    240607015455_mnzw ACCCCAGGGACATGCACCCCACCCCAGCTCTG(+ATATAT)GATCCAGCACCGTGTGTACCGGCTCCCACAT NGG 10 8 17 8 24 0 100 5 10
    240607015453_kgxe GAACGCCTACTTCCTGTCTCTGCTGTCTCCCG(+ATATAT)GTACCTGTGCTTCTGAGCCCAGACTATCTCT NGG 10 8 17 8 24 0 100 5 10
    240607015452_p6bd TGACAAGAGATAGTCTGGGCTCAGAAGCACAG(+ATATAT)GTACCGGGAGACAGCAGAGACAGGAAGTAGG NGG 10 8 17 8 24 0 100 5 10
    240607015450_xf3b AGCAGGATGCAAATGTGGAGGCAGTCCCAGAG(+ATATAT)GATCCCAAAACTGCTGCCAAGTTACAGGAGA NGG 10 8 17 8 24 0 100 5 10
    240607015449_61hj GGGTGTCTCCTGTAACTTGGCAGCAGTTTTGG(+ATATAT)GATCCTCTGGGACTGCCTCCACATTTGCATC NGG 10 8 17 8 24 0 100 5 10
    240607015447_k8nq GGACAATCCTGACCCTCTTCCGCTGGCCTGTG(+ATATAT)GATCCCTTGACAAGCGACTCTGTTCTGTCCA NGG 10 8 17 8 24 0 100 5 10