Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015429_7ods GTGGGGGATGGGAGACGGGGACTGGGGGGGGG(+ATATAT)GATCCATCCTTTTCTTTCCTCTTGGTGTTCG NGG 10 8 17 8 24 0 100 5 10
    240607015428_iaoh AAAATTTGAAAATATTGACTCCTGAGTGGGAG(+ATATAT)GTACCACACCTGAACCAACCCACAAACGCTG NGG 10 8 17 8 24 0 100 5 10
    240607015427_kww7 CCGGCCAGCGTTTGTGGGTTGGTTCAGGTGTG(+ATATAT)GTACCTCCCACTCAGGAGTCAATATTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240607015425_s62m AATGTATCAAATCATGAAGCAAGGTGGCCAAG(+ATATAT)GATCCCATAGCCTGGTCACAGTGGGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240607015423_0z8v GAGGTCTGCTTCCCACTGTGACCAGGCTATGG(+ATATAT)GATCCTTGGCCACCTTGCTTCATGATTTGAT NGG 10 8 17 8 24 0 100 5 10
    240607015422_rip7 ATTTTAGATGTTTAAGATAAGAAACTAGCTTG(+ATATAT)GTACCATTAAAATGCCCACTATGTCCTACTG NGG 10 8 17 8 24 0 100 5 10
    240607015421_jgyo GTGATCAGTAGGACATAGTGGGCATTTTAATG(+ATATAT)GTACCAAGCTAGTTTCTTATCTTAAACATCT NGG 10 8 17 8 24 0 100 5 10
    240607015419_wql2 CAGTTTTCCGCATGATGTCTTCTGTGATGCAG(+ATATAT)GTACCCTTATTCATTTGTGATTTTCAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240607015418_nrce GTGCTATTCTTGAAAATCACAAATGAATAAGG(+ATATAT)GTACCTGCATCACAGAAGACATCATGCGGAA NGG 10 8 17 8 24 0 100 5 10
    240607015417_ja44 TTTGCTATAGTCACTAGGGCCATTTACTATAG(+ATATAT)GATCCATTGTAATGAGAATGTTCATAAACTG NGG 10 8 17 8 24 0 100 5 10