Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015445_6tit GACCCTGGACAGAACAGAGTCGCTTGTCAAGG(+ATATAT)GATCCACAGGCCAGCGGAAGAGGGTCAGGAT NGG 10 8 17 8 24 0 100 5 10
    240607015442_2gv1 CTGCTGGCATCACCACACTCAGCCAGCCCCTG(+ATATAT)GATCCTTCTGACTTGGCTTTGCCATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240607015441_3j5x AAAACAAAAAAATGGCAAAGCCAAGTCAGAAG(+ATATAT)GATCCAGGGGCTGGCTGAGTGTGGTGATGCC NGG 10 8 17 8 24 0 100 5 10
    240607015440_vd1l AAGGAGGCAAGTCCTAACATGGGGGTGAGCTG(+ATATAT)GATCCAGACACGGGTGGGTGCGTAGAGTGCC NGG 10 8 17 8 24 0 100 5 10
    240607015439_7vt0 CTCTCGGCACTCTACGCACCCACCCGTGTCTG(+ATATAT)GATCCAGCTCACCCCCATGTTAGGACTTGCC NGG 10 8 17 8 24 0 100 5 10
    240607015437_omif GGGGCAGGCCAAAAGATGGATGATAAGAAGTG(+ATATAT)GATCCACTTGAGAGTGGCTGAAGAACCAAGG NGG 10 8 17 8 24 0 100 5 10
    240607015436_5r6m CGCTACCTTGGTTCTTCAGCCACTCTCAAGTG(+ATATAT)GATCCACTTCTTATCATCCATCTTTTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240607015435_fsq7 CTTTAAGGATGTGTTAAAATCTGAAGGCATTG(+ATATAT)GATCCTGAGGAATGATCAGGAGAGGCACTGA NGG 10 8 17 8 24 0 100 5 10
    240607015433_vfnk CCTGCTCAGTGCCTCTCCTGATCATTCCTCAG(+ATATAT)GATCCAATGCCTTCAGATTTTAACACATCCT NGG 10 8 17 8 24 0 100 5 10
    240607015431_fb8g AGCCGCGAACACCAAGAGGAAAGAAAAGGATG(+ATATAT)GATCCCCCCCCCCAGTCCCCGTCTCCCATCC NGG 10 8 17 8 24 0 100 5 10