Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015859_s3a7 CTCAAAATTGGGATATAGAATGAGCCTGTCAG(+ATATAT)GTACCTATCAGGAGCGGTAATCCGTGAGAGA NGG 10 8 17 8 24 0 100 5 10
    240607015857_xlwl GGACTAGAATCAAGGTTGGGGTGCCCCTCTGG(+ATATAT)GATCCTGACTGCTTGAGACTGAAGGGGCTGC NGG 10 8 17 8 24 0 100 5 10
    240607015856_ybw6 CCTCCGCAGCCCCTTCAGTCTCAAGCAGTCAG(+ATATAT)GATCCCAGAGGGGCACCCCAACCTTGATTCT NGG 10 8 17 8 24 0 100 5 10
    240607015855_d6ap TCTATCCATCACTTGGTAAATCGGAAAGGATG(+ATATAT)GATCCCGAGATCATTGTGATAGTGTTTATCA NGG 10 8 17 8 24 0 100 5 10
    240607015853_twgh ATTTTTGATAAACACTATCACAATGATCTCGG(+ATATAT)GATCCATCCTTTCCGATTTACCAAGTGATGG NGG 10 8 17 8 24 0 100 5 10
    240607015852_giir CAAGGACTCAGAACAACATGGCTTTTTGATCG(+ATATAT)GTACCAACCGACCTACATCAATTAGTTATTG NGG 10 8 17 8 24 0 100 5 10
    240607015851_50hg AAGTCCAATAACTAATTGATGTAGGTCGGTTG(+ATATAT)GTACCGATCAAAAAGCCATGTTGTTCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240607015849_u1g2 CCTTCTGTGGAAAAAAGTTTGAGTTTTGGTAG(+ATATAT)GTACCCTTGCAAGGAATTCCAACCTAGATGT NGG 10 8 17 8 24 0 100 5 10
    240607015848_fj8h TTCTAACATCTAGGTTGGAATTCCTTGCAAGG(+ATATAT)GTACCTACCAAAACTCAAACTTTTTTCCACA NGG 10 8 17 8 24 0 100 5 10
    240607015846_qii4 CTAACATTTAAGTGTTGGAAAGCTGAAAACAG(+ATATAT)GTACCTGAGAGTAACTAGCCAATAATACCCC NGG 10 8 17 8 24 0 100 5 10