Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015956_7ywl TCCCACTTCCTCCTCACTTCTTTTCCCCTCTG(+ATATAT)GATCCACTCCCTTTCTGTCCCATTAGGAAAG NGG 10 8 17 8 24 0 100 5 10
    240607015955_sq0v AGCATTTTGTCTGCATGTAATGGTACAGTCAG(+ATATAT)GTACCATTACAACTGGCTTATTAAATTCAAT NGG 10 8 17 8 24 0 100 5 10
    240607015954_oqca TCTACATTGAATTTAATAAGCCAGTTGTAATG(+ATATAT)GTACCTGACTGTACCATTACATGCAGACAAA NGG 10 8 17 8 24 0 100 5 10
    240607015953_z752 AAAAAAAGTTTCAGAAAAATGATTTTATCTTG(+ATATAT)GATCCATTTGTGAATACCTAGTGCAGCTTAT NGG 10 8 17 8 24 0 100 5 10
    240607015952_qm49 CCCTGATAAGCTGCACTAGGTATTCACAAATG(+ATATAT)GATCCAAGATAAAATCATTTTTCTGAAACTT NGG 10 8 17 8 24 0 100 5 10
    240607015950_gte6 ACTGTGGAATCTTCCTAGTTGTGCCTTCCAAG(+ATATAT)GATCCTTTCATCATGTGGGTGAGCATATGCA NGG 10 8 17 8 24 0 100 5 10
    240607015948_crk1 TGTAATGCATATGCTCACCCACATGATGAAAG(+ATATAT)GATCCTTGGAAGGCACAACTAGGAAGATTCC NGG 10 8 17 8 24 0 100 5 10
    240607015947_982u ACTGTGGAATCTTCCTAGTTGTGCCTTCCAAG(+ATATAT)GATCCTTTCATCATGTGGGTGAGCATATGCA NGG 10 8 17 8 24 0 100 5 10
    240607015946_5sdj TGTAATGCATATGCTCACCCACATGATGAAAG(+ATATAT)GATCCTTGGAAGGCACAACTAGGAAGATTCC NGG 10 8 17 8 24 0 100 5 10
    240607015944_3z9c GTATGGAAGGTTATATCCTACATACCCCAGCG(+ATATAT)GTACCCCTTGACAGTTTACCATGCATAGACA NGG 10 8 17 8 24 0 100 5 10