Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020026_vxjv TGCCAATCTCACCCTTGCCCGCTTGTCTGCAG(+ATATAT)GTACCCAGGAAGGCTTTTGACTTCTGCCCTA NGG 10 8 17 8 24 0 100 5 10
    240607020024_o2st AAGTGTGGAGGGGGGTGTACCCCTGAACACGG(+ATATAT)GATCCTGTGTGCAGCCAAATGAGTTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240607020023_8uho TGCCAGCCTGCAACTCATTTGGCTGCACACAG(+ATATAT)GATCCCGTGTTCAGGGGTACACCCCCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240607020022_uka6 AGAAGATGGAACCCTCACATTTGAGGGGCTTG(+ATATAT)GTACCTCAACTAGATAGCACCGGGGACCAAG NGG 10 8 17 8 24 0 100 5 10
    240607020021_vhdg TGATGCTTGGTCCCCGGTGCTATCTAGTTGAG(+ATATAT)GTACCAAGCCCCTCAAATGTGAGGGTTCCAT NGG 10 8 17 8 24 0 100 5 10
    240607020020_8rv0 GAGAGAGTTGGGCAGGGGTGGGGTGCAGAAAG(+ATATAT)GATCCCCTTGCACAAAATCTTTGGCACTGCA NGG 10 8 17 8 24 0 100 5 10
    240607020018_xtne TTACTTGCAGTGCCAAAGATTTTGTGCAAGGG(+ATATAT)GATCCTTTCTGCACCCCACCCCTGCCCAACT NGG 10 8 17 8 24 0 100 5 10
    240607020016_oy9j ATCAGGCTATAAGCTTTGTAACTGCTACTAAG(+ATATAT)GATCCAGGGTACACCCTGTCCTGCAGAGACA NGG 10 8 17 8 24 0 100 5 10
    240607020014_v5n7 ACAGCTGTCTCTGCAGGACAGGGTGTACCCTG(+ATATAT)GATCCTTAGTAGCAGTTACAAAGCTTATAGC NGG 10 8 17 8 24 0 100 5 10
    240607020012_cbda AAAATGTACAAAGTCTGAATAAAGCATTTTGG(+ATATAT)GATCCGCCATCCGGTCTCTGCATATACAGAT NGG 10 8 17 8 24 0 100 5 10