Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020042_eidw TGGGGCTTCCTGCATTCCTTCCTGAGATGGGG(+ATATAT)GATCCCCTCAAGACATCCCCGATCCTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240607020041_uawj TGAGGCCTGGCATTATTAGAGCATCTCACAGG(+ATATAT)GATCCTTTCTACATTAGATCAGAAGCCCAGG NGG 10 8 17 8 24 0 100 5 10
    240607020040_1r0t GGACCCCTGGGCTTCTGATCTAATGTAGAAAG(+ATATAT)GATCCCTGTGAGATGCTCTAATAATGCCAGG NGG 10 8 17 8 24 0 100 5 10
    240607020038_pjpk CTGCGAACCTACTCAGGGCTATTTGCTCTCTG(+ATATAT)GATCCCTGGTCTGCATTCACTCCATAAAAAT NGG 10 8 17 8 24 0 100 5 10
    240607020036_jqgp CCCACATTTTTATGGAGTGAATGCAGACCAGG(+ATATAT)GATCCAGAGAGCAAATAGCCCTGAGTAGGTT NGG 10 8 17 8 24 0 100 5 10
    240607020034_cctc CTACTGTAATCCCAGAAATTGGAATTCCTTGG(+ATATAT)GTACCCAAGTCCCCTGCTATAATTAGTAGGC NGG 10 8 17 8 24 0 100 5 10
    240607020033_m0c7 ATTGTGCCTACTAATTATAGCAGGGGACTTGG(+ATATAT)GTACCCAAGGAATTCCAATTTCTGGGATTAC NGG 10 8 17 8 24 0 100 5 10
    240607020031_xprf TGGACAAAGCGGGATGTGCGCAGAATCTAATG(+ATATAT)GATCCCGGCAAATCTTCCAGGTAGGAATATT NGG 10 8 17 8 24 0 100 5 10
    240607020029_ob43 GGAACAATATTCCTACCTGGAAGATTTGCCGG(+ATATAT)GATCCATTAGATTCTGCGCACATCCCGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607020027_wxy1 AGGACTAGGGCAGAAGTCAAAAGCCTTCCTGG(+ATATAT)GTACCTGCAGACAAGCGGGCAAGGGTGAGAT NGG 10 8 17 8 24 0 100 5 10