Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015942_gftr TTACATGTCTATGCATGGTAAACTGTCAAGGG(+ATATAT)GTACCGCTGGGGTATGTAGGATATAACCTTC NGG 10 8 17 8 24 0 100 5 10
    240607015941_kj2p CTGTCCATAGCCTGGCACCTCCTGAAGGACAG(+ATATAT)GATCCTGTGGGACGGGGCTGTGTTGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240607015939_9e4x GAGAGTGCAGACAACACAGCCCCGTCCCACAG(+ATATAT)GATCCTGTCCTTCAGGAGGTGCCAGGCTATG NGG 10 8 17 8 24 0 100 5 10
    240607015938_2yoe CACTTATTTCCATTGGGACTCAAGTGACCAAG(+ATATAT)GTACCCTTGAAAACTCTAAGCCTACAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240607015936_qt7w CACCTGAGGCTGTAGGCTTAGAGTTTTCAAGG(+ATATAT)GTACCTTGGTCACTTGAGTCCCAATGGAAAT NGG 10 8 17 8 24 0 100 5 10
    240607015934_bulf CATAAACAAGGAAGCCCAATCAATTTCAGAGG(+ATATAT)GATCCCATTACTTAAGTCCTTAAAGGTTGGT NGG 10 8 17 8 24 0 100 5 10
    240607015933_7bgc CATGAACCAACCTTTAAGGACTTAAGTAATGG(+ATATAT)GATCCCTCTGAAATTGATTGGGCTTCCTTGT NGG 10 8 17 8 24 0 100 5 10
    240607015931_cj4g GTTAAGGCTGGTCTGCTGGGCTACAAAGGGAG(+ATATAT)GTACCATTGCAGCCAACCCTTTACAGCTCTA NGG 10 8 17 8 24 0 100 5 10
    240607015930_924t ACATCTAGAGCTGTAAAGGGTTGGCTGCAATG(+ATATAT)GTACCTCCCTTTGTAGCCCAGCAGACCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607015929_2518 GCAGATGTGTGTGGCATGTGGCCAGTTCACAG(+ATATAT)GATCCTCCAGTTCTAAAACACACAGAGTACG NGG 10 8 17 8 24 0 100 5 10