Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607015928_ebep CTGCCCGTACTCTGTGTGTTTTAGAACTGGAG(+ATATAT)GATCCTGTGAACTGGCCACATGCCACACACA NGG 10 8 17 8 24 0 100 5 10
    240607015926_7db7 AATGAGCTACCCAACTTCTACCACATTCCTGG(+ATATAT)GTACCCAACTGTGTTGTTACTCTGAACCCAC NGG 10 8 17 8 24 0 100 5 10
    240607015924_ucbh AAACTGTGGGTTCAGAGTAACAACACAGTTGG(+ATATAT)GTACCCAGGAATGTGGTAGAAGTTGGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240607015923_7tlh GAAGAAGCCCAAGAAAAAAGCAGGGTTATCTG(+ATATAT)GTACCTATCCTGGGCAGAATCACAGGTTGTA NGG 10 8 17 8 24 0 100 5 10
    240607015922_t6om ATCAGTACAACCTGTGATTCTGCCCAGGATAG(+ATATAT)GTACCAGATAACCCTGCTTTTTTCTTGGGCT NGG 10 8 17 8 24 0 100 5 10
    240607015920_nhg5 CACACGTTAAGATGCCTTTCTCGCCTCTGAAG(+ATATAT)GATCCTGAGTCACTGCCCTCATTTACTATGA NGG 10 8 17 8 24 0 100 5 10
    240607015919_qxrm GGGATTCATAGTAAATGAGGGCAGTGACTCAG(+ATATAT)GATCCTTCAGAGGCGAGAAAGGCATCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240607015918_qidi TCAGTCTCAGCACTCAGAGACAGAGCTAGGTG(+ATATAT)GATCCCTGAGTTCGAGGCCAGCCTGGTCTAC NGG 10 8 17 8 24 0 100 5 10
    240607015916_bv37 TCTCTGTAGACCAGGCTGGCCTCGAACTCAGG(+ATATAT)GATCCACCTAGCTCTGTCTCTGAGTGCTGAG NGG 10 8 17 8 24 0 100 5 10
    240607015914_e4vi GTATTGTTTGTCAGCTCAGAAAGAATCAGGTG(+ATATAT)GTACCCAAGTGCGGGGTCTTCCTAACGTTGA NGG 10 8 17 8 24 0 100 5 10