Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020113_1t0c TGATGACCATATGCTGGTGACCTACAAATCGG(+ATATAT)GATCCTGCCATATGGGGTCGCCACTGGACTT NGG 10 8 17 8 24 0 100 5 10
    240607020111_jqsi GGGATGTAGCAGTAGCTTCCTCAGCAGCCTGG(+ATATAT)GATCCCAGAAGGAGGCCAATCAGTGACACTC NGG 10 8 17 8 24 0 100 5 10
    240607020110_vjce CACGCGAGTGTCACTGATTGGCCTCCTTCTGG(+ATATAT)GATCCCAGGCTGCTGAGGAAGCTACTGCTAC NGG 10 8 17 8 24 0 100 5 10
    240607020108_7mc3 GCCTGAACTGAACACAGGAGGAATGGTTTGGG(+ATATAT)GATCCTGCACCATTCCAGGATTGATTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240607020107_uga5 ATTCCAGAAAAATCAATCCTGGAATGGTGCAG(+ATATAT)GATCCCCAAACCATTCCTCCTGTGTTCAGTT NGG 10 8 17 8 24 0 100 5 10
    240607020104_s3ks ACATGGTAGCTCACAACGAGCACAACTCAGGG(+ATATAT)GATCCAGCATTCTCTGGCCTCTGTGTGTATG NGG 10 8 17 8 24 0 100 5 10
    240607020103_146k TCAGACATACACACAGAGGCCAGAGAATGCTG(+ATATAT)GATCCCCTGAGTTGTGCTCGTTGTGAGCTAC NGG 10 8 17 8 24 0 100 5 10
    240607020102_uwmt ATCATACCTGGAATGCATGCAGCCTGCTGTTG(+ATATAT)GTACCCTGCTCACCTGTGTCTTTCTTACCCA NGG 10 8 17 8 24 0 100 5 10
    240607020101_d2t7 CCTCCTGGGTAAGAAAGACACAGGTGAGCAGG(+ATATAT)GTACCAACAGCAGGCTGCATGCATTCCAGGT NGG 10 8 17 8 24 0 100 5 10
    240607020059_bp3a TAAACCCAGTCCTTAGGAGACTGAGGCAGGAG(+ATATAT)GATCCCTGTGAGTTCCAGGCCAGTCTAAGCT NGG 10 8 17 8 24 0 100 5 10