Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020203_5eu8 CTGGTCCCACGGGCAGGAGACAGCTGTGAGTG(+ATATAT)GATCCAGGTGCCCCCAAAGAGCGGCTTTCTC NGG 10 8 17 8 24 0 100 5 10
    240607020201_crhy GAAAGGAGGGGGGCCACAGGCAAGAAGACAGG(+ATATAT)GTACCCACGCATAGACTTTCCATCTCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240607020200_8a99 GCGTTGTTGGGAGATGGAAAGTCTATGCGTGG(+ATATAT)GTACCCTGTCTTCTTGCCTGTGGCCCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240607020158_u8ub GCCAAGTGGTATTTTGGAAACAGGGACATCGG(+ATATAT)GTACCCTACAGAGTGTAGACAAACACGTTTT NGG 10 8 17 8 24 0 100 5 10
    240607020157_5f6i GGGTCAAAACGTGTTTGTCTACACTCTGTAGG(+ATATAT)GTACCCGATGTCCCTGTTTCCAAAATACCAC NGG 10 8 17 8 24 0 100 5 10
    240607020155_gv6r GCCAAGTGGTATTTTGGAAACAGGGACATCGG(+ATATAT)GTACCCTACAGAGTGTAGACAAACACGTTTT NGG 10 8 17 8 24 0 100 5 10
    240607020154_cz8r GGGTCAAAACGTGTTTGTCTACACTCTGTAGG(+ATATAT)GTACCCGATGTCCCTGTTTCCAAAATACCAC NGG 10 8 17 8 24 0 100 5 10
    240607020152_ag12 TCCCTCCGCAGATCTAGTATAAGGGCAGCCTG(+ATATAT)GTACCCTTCTTCTTCCACAGAAGCCAGGTTA NGG 10 8 17 8 24 0 100 5 10
    240607020150_mn39 TAAGGTAACCTGGCTTCTGTGGAAGAAGAAGG(+ATATAT)GTACCAGGCTGCCCTTATACTAGATCTGCGG NGG 10 8 17 8 24 0 100 5 10
    240607020149_ywgn TCCCAGAATAAACAATCCAGGAAGGCCAGCTG(+ATATAT)GATCCAAGGGACTCAACGACCTCTCCAGGCA NGG 10 8 17 8 24 0 100 5 10