Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020305_ksdz AGTCCCCTGAGTAAAGAACACAGCTCAGGAAG(+ATATAT)GATCCAGGGAACCCTTTCTCAGAGCTGCTTG NGG 10 8 17 8 24 0 100 5 10
    240607020303_c4oh TGGTGGAAACATCCTTCCAACAAGTGTAGTGG(+ATATAT)GATCCCGGGGCCCAGGGCAGGCCGAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607020300_19cm GCAGGCCAGCCTCGGCCTGCCCTGGGCCCCGG(+ATATAT)GATCCCACTACACTTGTTGGAAGGATGTTTC NGG 10 8 17 8 24 0 100 5 10
    240607020258_5kk9 ATGGACTCCGAGGGCGTGGGGTGGGCTCCAAG(+ATATAT)GATCCTGGGCATCCGGATGGCATCGGCTACC NGG 10 8 17 8 24 0 100 5 10
    240607020256_9h9s TATGTGGTAGCCGATGCCATCCGGATGCCCAG(+ATATAT)GATCCTTGGAGCCCACCCCACGCCCTCGGAG NGG 10 8 17 8 24 0 100 5 10
    240607020255_uleo TTAATTCCAAGCCACACATGATGGTGGCACAG(+ATATAT)GATCCTAACCCCAGTGCTTGGAAGGCTACGG NGG 10 8 17 8 24 0 100 5 10
    240607020254_ahvk TGTAGCCGTAGCCTTCCAAGCACTGGGGTTAG(+ATATAT)GATCCTGTGCCACCATCATGTGTGGCTTGGA NGG 10 8 17 8 24 0 100 5 10
    240607020252_5pxl GGAGGGCTCCAGCGGGCAATGGGATGGATAGG(+ATATAT)GTACCTGCTCCCGAAGCTGTGGCAAAGTCTT NGG 10 8 17 8 24 0 100 5 10
    240607020251_vafd CTAGCAAGACTTTGCCACAGCTTCGGGAGCAG(+ATATAT)GTACCCTATCCATCCCATTGCCCGCTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240607020250_5vuy TAATGGAAGTGTTAATTTTACTGTACTTTTTG(+ATATAT)GTACCTTTTGGGAATCTAATGTATTGTAAGG NGG 10 8 17 8 24 0 100 5 10