Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020337_ggyz ATTAAGACTCCTTCAGAAAAGCTAGTACTTTG(+ATATAT)GTACCTGATTTAGTGTAATCAATTGGGTATA NGG 10 8 17 8 24 0 100 5 10
    240607020335_e5bs GGGAGAGGATGACTCTCTTGATGGTGTATGGG(+ATATAT)GATCCACAACTTGAGGGTCATTTGGCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240607020334_xfk3 CTATTGTGCTGCCAAATGACCCTCAAGTTGTG(+ATATAT)GATCCCCATACACCATCAAGAGAGTCATCCT NGG 10 8 17 8 24 0 100 5 10
    240607020332_607t ATGACTTCAGGCCTTTCCCAGCCTCCTCCCGG(+ATATAT)GATCCAGGAAGGCAGCAGGATATTTGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240607020330_24oa CCACATCACACAAATATCCTGCTGCCTTCCTG(+ATATAT)GATCCCGGGAGGAGGCTGGGAAAGGCCTGAA NGG 10 8 17 8 24 0 100 5 10
    240607020329_yl0x GCAACTGACAGAAGTGTCAGCCTGGGCAGCAG(+ATATAT)GATCCCTCGGTTCTGTGGTGTCCCCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607020327_t8lg CGTGTTGCCTGGGGGACACCACAGAACCGAGG(+ATATAT)GATCCTGCTGCCCAGGCTGACACTTCTGTCA NGG 10 8 17 8 24 0 100 5 10
    240607020325_g1mc GATTTGGAAGTGGGTGCACTGCCACACCTGTG(+ATATAT)GTACCAGCACTTGAGAAGCTGAGGCAGTAAG NGG 10 8 17 8 24 0 100 5 10
    240607020324_atj4 CCGGTCTTACTGCCTCAGCTTCTCAAGTGCTG(+ATATAT)GTACCACAGGTGTGGCAGTGCACCCACTTCC NGG 10 8 17 8 24 0 100 5 10
    240607020322_1ezz ATTAGATCTATGTAGATGTATCATCTTAAGGG(+ATATAT)GATCCTGCCGCCTCTGACAGCCATATCTCTT NGG 10 8 17 8 24 0 100 5 10