Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020439_7vxi TTGCTCTCTATTGCTCTTCCTCACACCAGATG(+ATATAT)GTACCTACGCCAGGAACCTTTGGAGAGCGCT NGG 10 8 17 8 24 0 100 5 10
    240607020437_zfjf TAGGGCCCAACAAGATGGTTAGACAGGTAAAG(+ATATAT)GTACCTGCAGACAACCTAATTTGATCCTTCG NGG 10 8 17 8 24 0 100 5 10
    240607020436_4e2n GGATTCGAAGGATCAAATTAGGTTGTCTGCAG(+ATATAT)GTACCTTTACCTGTCTAACCATCTTGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240607020435_767q AGTTTGAATCCATTCACAATGGAGCTTGCCTG(+ATATAT)GTACCTCTCAAGTGGCTTCCTGTAGTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240607020433_8sck TTGTCCCTCCACTACAGGAAGCCACTTGAGAG(+ATATAT)GTACCAGGCAAGCTCCATTGTGAATGGATTC NGG 10 8 17 8 24 0 100 5 10
    240607020432_gxzm CCACTGTTTGGTGAAGGTGCACCATAGGGCAG(+ATATAT)GTACCAGACTTAGGAGAAGGCCTCAGATCCC NGG 10 8 17 8 24 0 100 5 10
    240607020431_9kta CTGCTGGGATCTGAGGCCTTCTCCTAAGTCTG(+ATATAT)GTACCTGCCCTATGGTGCACCTTCACCAAAC NGG 10 8 17 8 24 0 100 5 10
    240607020429_m1z2 ATGGAAAGACAACACCAGACCCTTAGAGAATG(+ATATAT)GTACCTTTTCTCCACCTCCTTGAGGTCAAAG NGG 10 8 17 8 24 0 100 5 10
    240607020428_xxg2 ACGACCTTTGACCTCAAGGAGGTGGAGAAAAG(+ATATAT)GTACCATTCTCTAAGGGTCTGGTGTTGTCTT NGG 10 8 17 8 24 0 100 5 10
    240607020426_al2w ACGGGAGGACTCAAGACTCTTTGCAACCTTTG(+ATATAT)GATCCCTTGGACCACAGAGAGCTTTCAGATG NGG 10 8 17 8 24 0 100 5 10