Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020454_6p0g GTGGCTCACAGAACACATCTCTCAACCCAGGG(+ATATAT)GATCCAACCTCCCGACCCTGCACACAACCTA NGG 10 8 17 8 24 0 100 5 10
    240607020452_9cro TTCGGCACTAAGTGGCATCGGGGGGCGGGGAG(+ATATAT)GATCCCCAGGATGAGGACAGAGCCCAGCCCA NGG 10 8 17 8 24 0 100 5 10
    240607020450_o8q1 TACACTGGGCTGGGCTCTGTCCTCATCCTGGG(+ATATAT)GATCCTCCCCGCCCCCCGATGCCACTTAGTG NGG 10 8 17 8 24 0 100 5 10
    240607020448_zvcp ACGGAATGGGCACTTCATGCTGTTTAATGGAG(+ATATAT)GATCCCGCTGGGAACGCACTCAGGATTTATG NGG 10 8 17 8 24 0 100 5 10
    240607020446_6jl5 ACTTGCATAAATCCTGAGTGCGTTCCCAGCGG(+ATATAT)GATCCTCCATTAAACAGCATGAAGTGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240607020445_a7cl TTTGTGAAGTTAATAACGTCTACTTTTCTGTG(+ATATAT)GTACCATGGTCTTACCTTTCAAAACTTATGT NGG 10 8 17 8 24 0 100 5 10
    240607020444_1xao ATAGGACATAAGTTTTGAAAGGTAAGACCATG(+ATATAT)GTACCACAGAAAAGTAGACGTTATTAACTTC NGG 10 8 17 8 24 0 100 5 10
    240607020442_jliq TGCCTGAGACCTGAGAGAGGACTGAGAGATGG(+ATATAT)GTACCTCAGCTCTCAGCAGCATCATGTGCAT NGG 10 8 17 8 24 0 100 5 10
    240607020441_ioef ATTCAATGCACATGATGCTGCTGAGAGCTGAG(+ATATAT)GTACCCATCTCTCAGTCCTCTCTCAGGTCTC NGG 10 8 17 8 24 0 100 5 10
    240607020440_jcuu GCTTAAGCGCTCTCCAAAGGTTCCTGGCGTAG(+ATATAT)GTACCATCTGGTGTGAGGAAGAGCAATAGAG NGG 10 8 17 8 24 0 100 5 10