Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020510_yd0v CTGTCTGGAGGGGGGAGGGACGGTTCACAGTG(+ATATAT)GTACCAATCAGGAAGCTCTTGTGGCAGATGG NGG 10 8 17 8 24 0 100 5 10
    240607020509_8wui GTGCTTCAGGGTGGGCCCTATTTGGGCTTGAG(+ATATAT)GATCCCGTGGGGGCGGGGCTTCACCCCGACT NGG 10 8 17 8 24 0 100 5 10
    240607020507_aq7c CACCCAGTCGGGGTGAAGCCCCGCCCCCACGG(+ATATAT)GATCCTCAAGCCCAAATAGGGCCCACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240607020505_bhnb CTCTAGGAATCTCTATGGGGACTGCTAGGAAG(+ATATAT)GATCCTGGGCATGACAGCCTCGTATGATAGC NGG 10 8 17 8 24 0 100 5 10
    240607020503_32z4 TGCAGGCTATCATACGAGGCTGTCATGCCCAG(+ATATAT)GATCCTTCCTAGCAGTCCCCATAGAGATTCC NGG 10 8 17 8 24 0 100 5 10
    240607020502_0fwa ATAGATCCTAAGATCCAGCAAGCTTGGCTCAG(+ATATAT)GATCCTGTTGGGGAGCAGCCACCTGCCTTAT NGG 10 8 17 8 24 0 100 5 10
    240607020501_uyfy CTGGGATAAGGCAGGTGGCTGCTCCCCAACAG(+ATATAT)GATCCTGAGCCAAGCTTGCTGGATCTTAGGA NGG 10 8 17 8 24 0 100 5 10
    240607020459_zl11 GAGCTGAAGCCCCTTAGGAGGTGCTGGCTCAG(+ATATAT)GTACCTCCCTGCGGGATAGGAAGGGGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240607020458_idsp CCTGGTCACTCCCCTTCCTATCCCGCAGGGAG(+ATATAT)GTACCTGAGCCAGCACCTCCTAAGGGGCTTC NGG 10 8 17 8 24 0 100 5 10
    240607020456_gh8d TTGTATAGGTTGTGTGCAGGGTCGGGAGGTTG(+ATATAT)GATCCCCTGGGTTGAGAGATGTGTTCTGTGA NGG 10 8 17 8 24 0 100 5 10