Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020612_8cpx GTCTCCCCTACAAGGGGGGCTTTCTATTTTAG(+ATATAT)GTACCACCCCCTTAAACACACTGAAACAAAG NGG 10 8 17 8 24 0 100 5 10
    240607020610_6iev GCCCAAGATGGGCTTGCTGGGGAGCTAGGAGG(+ATATAT)GTACCAAGTAGAAGAGGGTATGGTTAGTTAT NGG 10 8 17 8 24 0 100 5 10
    240607020609_dzk6 CATAGATAACTAACCATACCCTCTTCTACTTG(+ATATAT)GTACCCTCCTAGCTCCCCAGCAAGCCCATCT NGG 10 8 17 8 24 0 100 5 10
    240607020608_qlm7 GTGCTGCCAGGCCAGGCCCTTTCATGACTGTG(+ATATAT)GATCCCCTATGCCTGACAGCCAGAGGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240607020606_bxad TAGCTGCCTTCCTCTGGCTGTCAGGCATAGGG(+ATATAT)GATCCACAGTCATGAAAGGGCCTGGCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240607020603_xf8e TGGAGAGCTGCTGACAGTTGGCAATACCCTGG(+ATATAT)GATCCACATTAGGGACCCCAGTGGACTGGAG NGG 10 8 17 8 24 0 100 5 10
    240607020601_rtvs GGCTCCTCCAGTCCACTGGGGTCCCTAATGTG(+ATATAT)GATCCCAGGGTATTGCCAACTGTCAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240607020600_t21h CTGCACATATGTCTGCATCACATGTGTACCTG(+ATATAT)GTACCCATGGACAACATCATAGAACTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240607020558_oeeq TTGTAACTCCAGTTCTATGATGTTGTCCATGG(+ATATAT)GTACCAGGTACACATGTGATGCAGACATATG NGG 10 8 17 8 24 0 100 5 10
    240607020556_iemm CTGCACATATGTCTGCATCACATGTGTACCTG(+ATATAT)GTACCCATGGACAACATCATAGAACTGGAGT NGG 10 8 17 8 24 0 100 5 10