Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020644_ms5y CAGATAGCACTCTGCTCTGGCGCTCTGACATG(+ATATAT)GTACCACACTGCTGCATGTGGAGGTTCAGGG NGG 10 8 17 8 24 0 100 5 10
    240607020642_55lt CATGAAAGAGTTATTAGCCACCAAGTATGCTG(+ATATAT)GTACCTATAATCCCAGAACACAGAAGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240607020641_eek1 CTGCCTCAGCCTTCTGTGTTCTGGGATTATAG(+ATATAT)GTACCAGCATACTTGGTGGCTAATAACTCTT NGG 10 8 17 8 24 0 100 5 10
    240607020640_7gbn GCATGAAGTTATCTGTGAGATGAGGGTAGGAG(+ATATAT)GATCCTGGGAGTCAGGACCACCCAGAACCAA NGG 10 8 17 8 24 0 100 5 10
    240607020637_dirt GTGGGTTGGTTCTGGGTGGTCCTGACTCCCAG(+ATATAT)GATCCTCCTACCCTCATCTCACAGATAACTT NGG 10 8 17 8 24 0 100 5 10
    240607020635_45a1 CCTGGAGGGCCTGGGCTGGAAATTTGCCCCTG(+ATATAT)GATCCAGAACCCCATTCGTTTTACAGTTGGG NGG 10 8 17 8 24 0 100 5 10
    240607020633_eief CCTATCCCAACTGTAAAACGAATGGGGTTCTG(+ATATAT)GATCCAGGGGCAAATTTCCAGCCCAGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240607020632_vlz7 TGCGTTCTTGCCATGAGCGAGCTGACTATCAG(+ATATAT)GTACCTGTTCTCGCTCTGTCTGGGAACTGTG NGG 10 8 17 8 24 0 100 5 10
    240607020631_fqdj TGTGACACAGTTCCCAGACAGAGCGAGAACAG(+ATATAT)GTACCTGATAGTCAGCTCGCTCATGGCAAGA NGG 10 8 17 8 24 0 100 5 10
    240607020629_o3vv GGCTAAGGATGGCCCTAAACATCCATCCTTCG(+ATATAT)GATCCTCCTGCCTCCTTTTCTCTAGTGCGGG NGG 10 8 17 8 24 0 100 5 10