Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020628_0n1i TGATCCCCGCACTAGAGAAAAGGAGGCAGGAG(+ATATAT)GATCCGAAGGATGGATGTTTAGGGCCATCCT NGG 10 8 17 8 24 0 100 5 10
    240607020627_z2v7 ACACCAACACATGAGCACAAGAGGCGGGTCAG(+ATATAT)GTACCCACACTCCACACTGTTTGGCTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240607020625_fi4a CCACATGAGAAGCCAAACAGTGTGGAGTGTGG(+ATATAT)GTACCTGACCCGCCTCTTGTGCTCATGTGTT NGG 10 8 17 8 24 0 100 5 10
    240607020623_qwjs TCTCCTAGCACAACAGTGCCAACAAGCTTCGG(+ATATAT)GATCCCAGCCCAGGTCTGGGGAACTTTGCCA NGG 10 8 17 8 24 0 100 5 10
    240607020621_g6us ACAGATGGCAAAGTTCCCCAGACCTGGGCTGG(+ATATAT)GATCCCGAAGCTTGTTGGCACTGTTGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240607020620_7z3x ACAAGGTAGCCCTCCACCAGGTCTTAGCGGCG(+ATATAT)GATCCCGTGCACTGCAATCCCCACCCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607020618_v7q7 ACACCTTCCTGGGTGGGGATTGCAGTGCACGG(+ATATAT)GATCCGCCGCTAAGACCTGGTGGAGGGCTAC NGG 10 8 17 8 24 0 100 5 10
    240607020617_cf39 GGGGAAGTCAGAGGTAATGCTCTGGATAAGAG(+ATATAT)GATCCAGTGGGCAGCTCCTGCCACGCACAGT NGG 10 8 17 8 24 0 100 5 10
    240607020615_2sbj TCACCACTGTGCGTGGCAGGAGCTGCCCACTG(+ATATAT)GATCCTCTTATCCAGAGCATTACCTCTGACT NGG 10 8 17 8 24 0 100 5 10
    240607020614_bjmi CATGGCTTTGTTTCAGTGTGTTTAAGGGGGTG(+ATATAT)GTACCTAAAATAGAAAGCCCCCCTTGTAGGG NGG 10 8 17 8 24 0 100 5 10