Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020712_l7ba ATTTGCATCTGCCAATGGATCTTGGTCCCTTG(+ATATAT)GATCCACTGACTGAGAAAAGTAATGGAGCTG NGG 10 8 17 8 24 0 100 5 10
    240607020710_r52t AAAAAGCAGCGGCAGACAGGATTTTTGTCCAG(+ATATAT)GATCCACTATCCAGCTTTAAGATGAGTCATT NGG 10 8 17 8 24 0 100 5 10
    240607020709_u4uy GGTCGAATGACTCATCTTAAAGCTGGATAGTG(+ATATAT)GATCCTGGACAAAAATCCTGTCTGCCGCTGC NGG 10 8 17 8 24 0 100 5 10
    240607020707_2p5x ATCTCTTTCGTGCAAACCACATTTCTACACTG(+ATATAT)GTACCTCCTAAGTCCAGAAGTTGGCCTCCGT NGG 10 8 17 8 24 0 100 5 10
    240607020706_h6vv TGGGCACGGAGGCCAACTTCTGGACTTAGGAG(+ATATAT)GTACCAGTGTAGAAATGTGGTTTGCACGAAA NGG 10 8 17 8 24 0 100 5 10
    240607020703_qqk4 GGTGTCCAGAGTGCCTGCAGACAGTGTGCCCG(+ATATAT)GTACCTCTGTCTACCTTTTGTAACGTTACCG NGG 10 8 17 8 24 0 100 5 10
    240607020702_i6sc CCAGGCGGTAACGTTACAAAAGGTAGACAGAG(+ATATAT)GTACCGGGCACACTGTCTGCAGGCACTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607020701_703o TACCACCCACCACGTATTACCCAAATACTCAG(+ATATAT)GATCCTACCAGCTACTGAGGCCACTGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240607020659_ea8o CCCATGCACACAGTGGCCTCAGTAGCTGGTAG(+ATATAT)GATCCTGAGTATTTGGGTAATACGTGGTGGG NGG 10 8 17 8 24 0 100 5 10
    240607020658_tapk CAGCACACTGAGAACATCTTAGTGAGTACACG(+ATATAT)GTACCTTAACTTGATATTTTTCAGCAAATGT NGG 10 8 17 8 24 0 100 5 10