Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020540_8ms8 AGATGACAACGGTTCCCCTGGCAGGCATGTAG(+ATATAT)GTACCTCTACTGTAGAATCTTGTCCAGCTTA NGG 10 8 17 8 24 0 100 5 10
    240607020539_cs39 TCAGACGTTGTTTTGACCAAAGTGTTCACTGG(+ATATAT)GATCCTCCAGGAAGTACCAGGGAACTGCAGC NGG 10 8 17 8 24 0 100 5 10
    240607020537_joeh GTTATGCTGCAGTTCCCTGGTACTTCCTGGAG(+ATATAT)GATCCCAGTGAACACTTTGGTCAAAACAACG NGG 10 8 17 8 24 0 100 5 10
    240607020536_0pwi CACATTAGTTAGTAACCACAGAACTGGTTTTG(+ATATAT)GATCCTGGAATTAAAGCATTAATATCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240607020534_z75z ATTTACCTTTGATATTAATGCTTTAATTCCAG(+ATATAT)GATCCAAAACCAGTTCTGTGGTTACTAACTA NGG 10 8 17 8 24 0 100 5 10
    240607020533_4cge GTGTCAGTCTCCAAGCTTACCTTTTATACAAG(+ATATAT)GATCCCAGTCTTGGGTTGGGGCTCACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240607020532_sqeg AGTAGACAGGGTGAGCCCCAACCCAAGACTGG(+ATATAT)GATCCTTGTATAAAAGGTAAGCTTGGAGACT NGG 10 8 17 8 24 0 100 5 10
    240607020530_rfjx GCTATTTCTTGCTCCAGGAGCATGGCTACCAG(+ATATAT)GATCCCCAATATTCAGCATCAACTGTTTTCA NGG 10 8 17 8 24 0 100 5 10
    240607020528_uj1r CCTGGTGAAAACAGTTGATGCTGAATATTGGG(+ATATAT)GATCCTGGTAGCCATGCTCCTGGAGCAAGAA NGG 10 8 17 8 24 0 100 5 10
    240607020527_wtzl CTGATACCTTCTTCAGCTCTTATTTAAGGAAG(+ATATAT)GTACCGTTTATGTTTCAAAACTTAGATATTC NGG 10 8 17 8 24 0 100 5 10