Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020320_42u7 ATAACAAGAGATATGGCTGTCAGAGGCGGCAG(+ATATAT)GATCCCCTTAAGATGATACATCTACATAGAT NGG 10 8 17 8 24 0 100 5 10
    240607020319_w6lm GCAGAAGGGGCGTGTTCAGATCTATGAAGTAG(+ATATAT)GTACCAGTTAGCACAAACCCAGGTCTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240607020318_yhcm CCTCTCTTCCAGACCTGGGTTTGTGCTAACTG(+ATATAT)GTACCTACTTCATAGATCTGAACACGCCCCT NGG 10 8 17 8 24 0 100 5 10
    240607020317_kkq2 CTACACACGGCTCCAGGTTATTCCTGTTGATG(+ATATAT)GATCCAAGGTGATCCAAATCATAAAGGCACC NGG 10 8 17 8 24 0 100 5 10
    240607020315_3r55 ACCTTGGTGCCTTTATGATTTGGATCACCTTG(+ATATAT)GATCCATCAACAGGAATAACCTGGAGCCGTG NGG 10 8 17 8 24 0 100 5 10
    240607020313_9hnn CATGAGTTAATAGCTTTCTTTAGGGGAGAAAG(+ATATAT)GATCCACAGGCAAAAGGAGAATCTTCCAAAG NGG 10 8 17 8 24 0 100 5 10
    240607020312_amvb ACGCGCTTTGGAAGATTCTCCTTTTGCCTGTG(+ATATAT)GATCCTTTCTCCCCTAAAGAAAGCTATTAAC NGG 10 8 17 8 24 0 100 5 10
    240607020310_zjwg GAGTGCTGTGCAGGTGTATGTGCAATGCATGG(+ATATAT)GTACCCTATGAAACTTGTTGGGGTTTCTAGA NGG 10 8 17 8 24 0 100 5 10
    240607020309_9khr GAATCTCTAGAAACCCCAACAAGTTTCATAGG(+ATATAT)GTACCCATGCATTGCACATACACCTGCACAG NGG 10 8 17 8 24 0 100 5 10
    240607020306_pedf CAGCCCAAGCAGCTCTGAGAAAGGGTTCCCTG(+ATATAT)GATCCTTCCTGAGCTGTGTTCTTTACTCAGG NGG 10 8 17 8 24 0 100 5 10