Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607020235_7ktv AGCCAAGGGATGGCACCACCCACAAATGGCTG(+ATATAT)GATCCTCCCCTATCAATCACTAATTAGGAAA NGG 10 8 17 8 24 0 100 5 10
    240607020233_vi4c GCCACAGATGGTACATCTAGTGGTCCAGATAG(+ATATAT)GATCCCTCCTGAAATCTCCTACTTCTTGTCT NGG 10 8 17 8 24 0 100 5 10
    240607020232_aj6i GTGGCAGACAAGAAGTAGGAGATTTCAGGAGG(+ATATAT)GATCCTATCTGGACCACTAGATGTACCATCT NGG 10 8 17 8 24 0 100 5 10
    240607020230_xu07 TCTACCAAAAACAGGGCTAAGGGCTGGAGATG(+ATATAT)GATCCCCGTTCCTTGTCCAGCCTCCTGCTAC NGG 10 8 17 8 24 0 100 5 10
    240607020228_plmz CAGCGGTAGCAGGAGGCTGGACAAGGAACGGG(+ATATAT)GATCCATCTCCAGCCCTTAGCCCTGTTTTTG NGG 10 8 17 8 24 0 100 5 10
    240607020226_ggf2 GTGCATATGCTGACGCCTGAGAACAGACCCAG(+ATATAT)GTACCCGTGGCTGAGCTGAGCTTCCTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240607020224_lz5j GGGAAAGCAGAGGAAGCTCAGCTCAGCCACGG(+ATATAT)GTACCTGGGTCTGTTCTCAGGCGTCAGCATA NGG 10 8 17 8 24 0 100 5 10
    240607020223_d8vq GACATGCCACCCACCGCACTTGGCTTTATCAG(+ATATAT)GATCCATTTGTTGGTAGAAGCAAAAGCAGCT NGG 10 8 17 8 24 0 100 5 10
    240607020222_ae7f CCAAGAGCTGCTTTTGCTTCTACCAACAAATG(+ATATAT)GATCCTGATAAAGCCAAGTGCGGTGGGTGGC NGG 10 8 17 8 24 0 100 5 10
    240607020221_c0mr CCTTTTCTGTGTGCTGTACATCCCAGTCAGTG(+ATATAT)GTACCTAGCCCAGTTTCTCTCTGGCTGGCAC NGG 10 8 17 8 24 0 100 5 10