Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012650_x5fr GTTTCAACTATTTATCAGATAGATTTGGTATG(+ATATAT)GATCCACATGTGGGCTGTGCTGTACTGTTAA NGG 10 8 17 8 24 0 100 5 10
    240607012649_g1g5 GGAGAGCCCTGCTGCTTTCTGATGTTTGGAAG(+ATATAT)GTACCCAGCATCCTGATTCCCTCCAGTTCAT NGG 10 8 17 8 24 0 100 5 10
    240607012647_6dhy GCCAAATGAACTGGAGGGAATCAGGATGCTGG(+ATATAT)GTACCTTCCAAACATCAGAAAGCAGCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240607012645_h4m6 GTCGGGCCCCCAACAACTTGAAGCTTCCTCTG(+ATATAT)GATCCTCACTTGAGCTCTTTGGGGATGGGGC NGG 10 8 17 8 24 0 100 5 10
    240607012644_52hn CCTAGGCCCCATCCCCAAAGAGCTCAAGTGAG(+ATATAT)GATCCAGAGGAAGCTTCAAGTTGTTGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240607012643_lx3s CTATGCTACCCCACTTCCTGTATGCTCAACTG(+ATATAT)GATCCTCAAGACCATTTCATCTCTAGCTGCA NGG 10 8 17 8 24 0 100 5 10
    240607012641_tb1l CGTGGTGCAGCTAGAGATGAAATGGTCTTGAG(+ATATAT)GATCCAGTTGAGCATACAGGAAGTGGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240607012640_cjp3 TGACAGACTTGATGCTTTGAAATTGACCATTG(+ATATAT)GTACCTCCTCCTTAAAAGAGAAGTGTCGTGT NGG 10 8 17 8 24 0 100 5 10
    240607012638_bwd6 CCTGAACACGACACTTCTCTTTTAAGGAGGAG(+ATATAT)GTACCAATGGTCAATTTCAAAGCATCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240607012637_vue3 CTGGGTGTGCAGGAATTCTGCCTGTGAAGTGG(+ATATAT)GATCCACAGCATGTTCGACGGGTGGGAGAAC NGG 10 8 17 8 24 0 100 5 10