Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012719_j5ic CATAGTTAACCCTCACTTGCAGAGATTCTGTG(+ATATAT)GATCCAAGTAGCAGCTACCCCTGTTAATCTG NGG 10 8 17 8 24 0 100 5 10
    240607012718_l6is CAGCCCCAGCAAGCGCCCAGGAACAGGGAGAG(+ATATAT)GATCCATGCATGTGAGCAGCCAGCATCAAGG NGG 10 8 17 8 24 0 100 5 10
    240607012717_wp4q GGTGTCCTTGATGCTGGCTGCTCACATGCATG(+ATATAT)GATCCTCTCCCTGTTCCTGGGCGCTTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607012716_hlux TTGATATAAAGCACAAACTGGCTTTAAACTCG(+ATATAT)GATCCTTCGGCCTCAGCCTTTCTAGCACCAA NGG 10 8 17 8 24 0 100 5 10
    240607012714_1b7v TCTCCTTGGTGCTAGAAAGGCTGAGGCCGAAG(+ATATAT)GATCCGAGTTTAAAGCCAGTTTGTGCTTTAT NGG 10 8 17 8 24 0 100 5 10
    240607012712_fh59 TGTGGGTAGAAAATGGTAACACTAAACAATGG(+ATATAT)GATCCCAGTACTCAACTTTAAAGATTTTATT NGG 10 8 17 8 24 0 100 5 10
    240607012711_hcsa GAGAAAATAAAATCTTTAAAGTTGAGTACTGG(+ATATAT)GATCCCATTGTTTAGTGTTACCATTTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240607012709_xuog ACACCCCTGAAACTGACAAGTGAGGGGCCAGG(+ATATAT)GATCCCCCACTGACTGGATTGTGTCTGGGCA NGG 10 8 17 8 24 0 100 5 10
    240607012707_797o TGATGTGCCCAGACACAATCCAGTCAGTGGGG(+ATATAT)GATCCCTGGCCCCTCACTTGTCAGTTTCAGG NGG 10 8 17 8 24 0 100 5 10
    240607012705_k78f AGGAAAAACCTCGCCTTTGACCTGAGAATGGG(+ATATAT)GTACCGCCCCTCGGGAACACAGTGAGCTCAC NGG 10 8 17 8 24 0 100 5 10