Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012749_0w34 GTAAACCAAAGAAGTGAAATAAATCTGAGCAG(+ATATAT)GTACCACAGGTGTGACACACGACACTCAACC NGG 10 8 17 8 24 0 100 5 10
    240607012747_77mb AGCCGAACCACAGATGGCTTCGAGTAAGTCTG(+ATATAT)GATCCTGACACCCGCCCTCCCCACCCCGAGC NGG 10 8 17 8 24 0 100 5 10
    240607012746_7dj0 CTGAAGCTCGGGGTGGGGAGGGCGGGTGTCAG(+ATATAT)GATCCAGACTTACTCGAAGCCATCTGTGGTT NGG 10 8 17 8 24 0 100 5 10
    240607012744_qkre CTGGTAAACTTGCCTTCCCGTTTCTCTGCCTG(+ATATAT)GTACCTTATTTGAGCAGGCTGTCAGCAATGG NGG 10 8 17 8 24 0 100 5 10
    240607012743_owt3 AAAGGCCATTGCTGACAGCCTGCTCAAATAAG(+ATATAT)GTACCAGGCAGAGAAACGGGAAGGCAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240607012742_fxby CTTGCAAAGTGCTCAGGAACACAGAATGTCTG(+ATATAT)GATCCTGATGTCAAGCAGGGTCCGGGAGCTT NGG 10 8 17 8 24 0 100 5 10
    240607012740_r2gv TGTGCAAGCTCCCGGACCCTGCTTGACATCAG(+ATATAT)GATCCAGACATTCTGTGTTCCTGAGCACTTT NGG 10 8 17 8 24 0 100 5 10
    240607012739_u02v TTCCTACTTGAAGCCCTTGCCCGTTCCAGCTG(+ATATAT)GATCCTTGGTGAGGACTACCGAGGTTTAACC NGG 10 8 17 8 24 0 100 5 10
    240607012737_x1kp GTGGAGGTTAAACCTCGGTAGTCCTCACCAAG(+ATATAT)GATCCAGCTGGAACGGGCAAGGGCTTCAAGT NGG 10 8 17 8 24 0 100 5 10
    240607012736_plt9 GTGCTCATCACCCACATGGCCAATCCAGATGG(+ATATAT)GTACCTAGTTATTCTGGCTCCCTGAGGGTCT NGG 10 8 17 8 24 0 100 5 10