Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012849_b9r1 TTGCAATTCCTGAAGGCTCGGTTTTGTTCCTG(+ATATAT)GTACCTTTGTTTTGTGGCAGGGACCTCCCCT NGG 10 8 17 8 24 0 100 5 10
    240607012848_e44o GTTCTGTCATTTGCTTTCTAAAGCAGTGCCAG(+ATATAT)GTACCATGGAATGTTCACGGCTCCAAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240607012846_phhp GGTGGTTCCTTTGGAGCCGTGAACATTCCATG(+ATATAT)GTACCTGGCACTGCTTTAGAAAGCAAATGAC NGG 10 8 17 8 24 0 100 5 10
    240607012845_nxpt ACAAAGGATGAGCCCTGCTGTGTTGCAAGTAG(+ATATAT)GATCCCCTCCCCCAGGTCTATGAGGCTAATA NGG 10 8 17 8 24 0 100 5 10
    240607012843_qdqo AATCTTATTAGCCTCATAGACCTGGGGGAGGG(+ATATAT)GATCCTACTTGCAACACAGCAGGGCTCATCC NGG 10 8 17 8 24 0 100 5 10
    240607012841_w1bu AACAGATGAAATCTTTCATGGCTGAAATCTTG(+ATATAT)GATCCCTAAATGACCCGCCACCGTGGGCTTT NGG 10 8 17 8 24 0 100 5 10
    240607012840_s6te TTATAAAAGCCCACGGTGGCGGGTCATTTAGG(+ATATAT)GATCCAAGATTTCAGCCATGAAAGATTTCAT NGG 10 8 17 8 24 0 100 5 10
    240607012839_plke AGAGCATCCCTCTGGAGGTAAGGTCCTCTCAG(+ATATAT)GTACCTTCTCACACCATCCCCAGTTACACCA NGG 10 8 17 8 24 0 100 5 10
    240607012837_2d02 GTTTCTGGTGTAACTGGGGATGGTGTGAGAAG(+ATATAT)GTACCTGAGAGGACCTTACCTCCAGAGGGAT NGG 10 8 17 8 24 0 100 5 10
    240607012835_qt8z AGAAAATCTTGCAAAATACATGGTTGCTTCAG(+ATATAT)GTACCAGCTCTTTACAATACAGTTCAAACTC NGG 10 8 17 8 24 0 100 5 10