Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607012939_mtob AGCAAGGCTCTCTCTTCTGGGCAAGGGCAATG(+ATATAT)GATCCTCAGAGGCCCCGGCAGAAGCCTAGCT NGG 10 8 17 8 24 0 100 5 10
    240607012937_yg1i AGAGAAGCTAGGCTTCTGCCGGGGCCTCTGAG(+ATATAT)GATCCATTGCCCTTGCCCAGAAGAGAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240607012936_o3ba GTCTTCACACTCTCCTGTATGTATCTGTATTG(+ATATAT)GTACCAATGAAATACAGTTCAGCATTTAATA NGG 10 8 17 8 24 0 100 5 10
    240607012935_r8ro TAGCATATTAAATGCTGAACTGTATTTCATTG(+ATATAT)GTACCAATACAGATACATACAGGAGAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240607012933_lkqn TCTCCCAACTATTCATAATTCTAGTTCAAGTG(+ATATAT)GATCCAGCACCCTCTTCTAACTGATACTGCC NGG 10 8 17 8 24 0 100 5 10
    240607012932_rado GTACTGGCAGTATCAGTTAGAAGAGGGTGCTG(+ATATAT)GATCCACTTGAACTAGAATTATGAATAGTTG NGG 10 8 17 8 24 0 100 5 10
    240607012929_lg1a TTTGTAGACCAGGCTGGCCTTGAAGTCCCAGG(+ATATAT)GATCCTGTCTGTGCCTCTCACATGTGTTAAG NGG 10 8 17 8 24 0 100 5 10
    240607012928_tc1j TTAGTCTTAACACATGTGAGAGGCACAGACAG(+ATATAT)GATCCCTGGGACTTCAAGGCCAGCCTGGTCT NGG 10 8 17 8 24 0 100 5 10
    240607012927_jk7i TCCTGGCAGGTGATCTACAAAGCAGAAGCGTG(+ATATAT)GTACCCAGTCAGTCACAGAGAATAGCATCCT NGG 10 8 17 8 24 0 100 5 10
    240607012925_7lwz AATCAAGGATGCTATTCTCTGTGACTGACTGG(+ATATAT)GTACCACGCTTCTGCTTTGTAGATCACCTGC NGG 10 8 17 8 24 0 100 5 10